Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat.
Expression profile of the entire family of Adhesion G protein-coupled receptors in mouse and rat.
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DOI：
10.1186/1471-2202-9-43
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发表时间：
2008-04-29
期刊：
影响因子：
2.4
通讯作者：
Fredriksson, Robert
中科院分区：
文献类型：
--
作者：
Haitina, Tatjana;Olsson, Fredrik;Stephansson, Olga;Alsio, Johan;Roman, Erika;Ebendal, Ted;Schioth, Helgi B.;Fredriksson, Robert
粘附性G蛋白偶联受体(GPCRs)是一种N末端较长的膜结合型受体。这个家庭在人类中有33个成员。已知一些粘附性GPCRs在中枢神经系统发育和大细胞表面配体介导的免疫系统反应中具有重要的生理功能。然而，大多数粘附性GPCR仍然是功能未知的孤儿，研究很少。在这项研究中，我们对大鼠和小鼠的整个粘附性GPCR家族进行了广泛的组织定位分析。通过应用实时定量聚合酶链式反应技术，我们已经为粘附性家族中的每个成员制作了可比较的表达谱。研究结果与文献数据和艾伦大脑图谱项目的数据进行了比较。我们的结果表明，大多数粘附性GPCRs要么在中枢神经系统中表达，要么在普遍存在。此外，来自同一系统发育组的粘附性GPCR具有主要的中枢或外周表达，尽管它们的每个表达谱都是独特的。我们的发现表明，许多粘附性GPCR在中枢神经系统中都有表达，而且很可能具有功能。相关的粘附性GPCR在结构上非常保守，有趣的是，它们的表达谱有相当大的重叠，这表明在许多系统发育相关的簇中，成员的生理作用相似。
The Adhesion G protein-coupled receptors (GPCRs) are membrane-bound receptors with long N termini. This family has 33 members in humans. Several Adhesion GPCRs are known to have important physiological functions in CNS development and immune system response mediated by large cell surface ligands. However, the majority of Adhesion GPCRs are still poorly studied orphans with unknown functions. In this study we performed the extensive tissue localization analysis of the entire Adhesion GPCR family in rat and mouse. By applying the quantitative real-time PCR technique we have produced comparable expression profile for each of the members in the Adhesion family. The results are compared with literature data and data from the Allen Brain Atlas project. Our results suggest that the majority of the Adhesion GPCRs are either expressed in the CNS or ubiquitously. In addition the Adhesion GPCRs from the same phylogenetic group have either predominant CNS or peripheral expression, although each of their expression profile is unique. Our findings indicate that many of Adhesion GPCRs are expressed, and most probably, have function in CNS. The related Adhesion GPCRs are well conserved in their structure and interestingly have considerable overlap in their expression profiles, suggesting similarities among the physiological roles for members within many of the phylogenetically related clusters.
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影响因子：
--
作者：
Jaspars, LH;Vos, W;Hamann, J
通讯作者：
Hamann, J
影响因子：
4.7
作者：
Lagerstrom, Malin C.;Rabe, Nadine;Schioth, Helgi B.
通讯作者：
Schioth, Helgi B.
DOI：
10.1073/pnas.0700901104
发表时间：
2007-09-04
影响因子：
11.1
作者：
Inoue, Haruhisa;Lin, Ling;Isacson, Ole
通讯作者：
Isacson, Ole
影响因子：
5.3
作者：
Davies, B;Baumann, C;Gottwald, U
通讯作者：
Gottwald, U
影响因子：
3.3
作者：
Little, KD;Hemler, ME;Stipp, CS
通讯作者：
Stipp, CS