Low Microbial Diversity and Abnormal Microbial Succession Is Associated with Necrotizing Enterocolitis in Preterm Infants.
Low Microbial Diversity and Abnormal Microbial Succession Is Associated with Necrotizing Enterocolitis in Preterm Infants.
复制标题
DOI：
10.3389/fmicb.2017.02243
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Roesch LFW
中科院分区：
文献类型：
--
作者：
Dobbler PT;Procianoy RS;Mai V;Silveira RC;Corso AL;Rojas BS;Roesch LFW
尽管加强了努力，坏死性小肠结肠炎(NEC)的不同病因在很大程度上仍然难以捉摸。NEC的临床预测因素仍然不明确，目前缺乏足够的特异性。对早产儿肠道微生物区系初始定植模式的深入了解可能有助于提高对NEC及其相关疾病的早期发现或预测。在这里，我们比较了患有NEC的新生儿和早产儿的粪便微生物区系演替、微生物多样性、丰度和结构。对40例早产儿(29例对照组和11例NEC病例)进行了基于16S rRNA的微生物区系分析，其中包括出生5周前的第一次粪便(胎粪)或NEC诊断。与肠杆菌科家族最接近的单一系统型匹配与NEC有很强的相关性。在这个系统类型最丰富的样本的DNA中，额外的猎枪元基因组测序显示，柯萨里柠檬酸杆菌和肺炎克雷伯氏菌是主要的分类群。这两个分类群可能是NEC早期诊断的合适的微生物标志物。在NEC病例中，在NEC诊断之前，我们进一步检测到微生物多样性较低和微生物群落的异常演替。最后，我们还检测到NEC病例胎粪样本的共生网络中厌氧微生物的破坏。我们的数据表明，克氏柠檬酸杆菌和/或肺炎克雷伯菌的强大优势，低多样性，低乳酸菌丰度，以及生命早期微生物网络结构的改变，与早产儿发生NEC的风险相关。在其他医院确认这些发现可能有助于开发基于微生物区系的筛查方法，以早期发现NEC。
Despite increased efforts, the diverse etiologies of Necrotizing Enterocolitis (NEC) have remained largely elusive. Clinical predictors of NEC remain ill-defined and currently lack sufficient specificity. The development of a thorough understanding of initial gut microbiota colonization pattern in preterm infants might help to improve early detection or prediction of NEC and its associated morbidities. Here we compared the fecal microbiota successions, microbial diversity, abundance and structure of newborns that developed NEC with preterm controls. A 16S rRNA based microbiota analysis was conducted in a total of 132 fecal samples that included the first stool (meconium) up until the 5th week of life or NEC diagnosis from 40 preterm babies (29 controls and 11 NEC cases). A single phylotype matching closest to the Enterobacteriaceae family correlated strongly with NEC. In DNA from the sample with the greatest abundance of this phylotype additional shotgun metagenomic sequencing revealed Citrobacter koseri and Klebsiella pneumoniae as the dominating taxa. These two taxa might represent suitable microbial biomarker targets for early diagnosis of NEC. In NEC cases, we further detected lower microbial diversity and an abnormal succession of the microbial community before NEC diagnosis. Finally, we also detected a disruption in anaerobic microorganisms in the co-occurrence network of meconium samples from NEC cases. Our data suggest that a strong dominance of Citrobacter koseri and/or Klebsiella pneumoniae, low diversity, low abundance of Lactobacillus, as well as an altered microbial-network structure during the first days of life, correlate with NEC risk in preterm infants. Confirmation of these findings in other hospitals might facilitate the development of a microbiota based screening approach for early detection of NEC.
登录
查看更多内容
影响因子：
3.7
作者：
Hu J;Nomura Y;Bashir A;Fernandez-Hernandez H;Itzkowitz S;Pei Z;Stone J;Loudon H;Peter I
通讯作者：
Peter I
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1136/fetalneonatal-2011-301373
发表时间：
2012-11
期刊：
Archives of disease in childhood. Fetal and neonatal edition
影响因子：
--
作者：
Madan JC;Salari RC;Saxena D;Davidson L;O'Toole GA;Moore JH;Sogin ML;Foster JA;Edwards WH;Palumbo P;Hibberd PL
通讯作者：
Hibberd PL
影响因子：
2.4
作者：
Dicken, Bryan J.;Sergi, Consolato;Sigalet, David
通讯作者：
Sigalet, David
影响因子：
2.3
作者：
Bezirtzoglou, Eugenia;Tsiotsias, Arsenis;Welling, Gjalt W.
通讯作者：
Welling, Gjalt W.