Diversified microbiota of meconium is affected by maternal diabetes status.
Diversified microbiota of meconium is affected by maternal diabetes status.
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DOI：
10.1371/journal.pone.0078257
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Peter I
中科院分区：
文献类型：
--
作者：
Hu J;Nomura Y;Bashir A;Fernandez-Hernandez H;Itzkowitz S;Pei Z;Stone J;Loudon H;Peter I
这项研究旨在评估胎粪微生物组的多样性，并确定细菌群落是否受到母体糖尿病状态的影响。收集了23例新生儿的首次肠道分泌物（胎粪），按母亲糖尿病状态分层：4例母亲患有妊娠前2型糖尿病（DM），其中1例母亲患有双卵双胞胎，5例发生妊娠期糖尿病（GDM），13例无糖尿病。使用多条形码16S rRNA测序分析胎粪微生物组，然后进行分类分配和多样性分析。所有的胎粪样本都不是无菌的，并且含有多样化的微生物群。与成人粪便相比，胎粪的物种多样性较低，样品间变异较大，变形菌门富集，拟杆菌门减少。在胎粪样本中，分类学分析表明，母体糖尿病状态的总体细菌含量显著不同，DM组的微生物组显示出比非糖尿病或GDM组更高的α多样性。阴道分娩与剖腹产婴儿之间没有全球差异。回归分析表明，胎粪微生物群组成的最可靠预测因素是妊娠前的母体糖尿病状态。具体而言，拟杆菌（门）和副拟杆菌（属）富集在胎粪中的DM组相比，非糖尿病组。我们的研究提供了证据，表明胎粪含有多样化的微生物群，并且不受分娩方式的影响。它还表明，患有DM的母亲所生婴儿的胎粪微生物组富含与成年DM患者粪便微生物组中报告的相同的细菌分类群。
This study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status. The first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers had pre-gestational type 2 diabetes mellitus (DM) including one mother with dizygotic twins, 5 developed gestational diabetes mellitus (GDM) and 13 had no diabetes. The meconium microbiome was profiled using multi-barcode 16S rRNA sequencing followed by taxonomic assignment and diversity analysis. All meconium samples were not sterile and contained diversified microbiota. Compared with adult feces, the meconium showed a lower species diversity, higher sample-to-sample variation, and enrichment of Proteobacteria and reduction of Bacteroidetes. Among the meconium samples, the taxonomy analyses suggested that the overall bacterial content significantly differed by maternal diabetes status, with the microbiome of the DM group showing higher alpha-diversity than that of no-diabetes or GDM groups. No global difference was found between babies delivered vaginally versus via Cesarean-section. Regression analysis showed that the most robust predictor for the meconium microbiota composition was the maternal diabetes status that preceded pregnancy. Specifically, Bacteroidetes (phyla) and Parabacteriodes (genus) were enriched in the meconium in the DM group compared to the no-diabetes group. Our study provides evidence that meconium contains diversified microbiota and is not affected by the mode of delivery. It also suggests that the meconium microbiome of infants born to mothers with DM is enriched for the same bacterial taxa as those reported in the fecal microbiome of adult DM patients.
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影响因子：
16.2
作者：
Jeon CY;Haan MN;Cheng C;Clayton ER;Mayeda ER;Miller JW;Aiello AE
通讯作者：
Aiello AE
使用通用的unifrac距离将微生物组组成与环境协变量相关联。
DOI：
10.1093/bioinformatics/bts342
发表时间：
2012-08-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Chen J;Bittinger K;Charlson ES;Hoffmann C;Lewis J;Wu GD;Collman RG;Bushman FD;Li H
通讯作者：
Li H
影响因子：
3.7
作者：
Hong PY;Lee BW;Aw M;Shek LP;Yap GC;Chua KY;Liu WT
通讯作者：
Liu WT
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
3.5
作者：
Dunne, F;Brydon, P;Gee, H
通讯作者：
Gee, H