Construction of chemiluminescence aptasensor platform using magnetic microsphere for ochratoxin A detection based on G bases derivative reaction and Au NPs catalyzing luminol system
Construction of chemiluminescence aptasensor platform using magnetic microsphere for ochratoxin A detection based on G bases derivative reaction and Au NPs catalyzing luminol system
复制标题
基于G碱基衍生反应和金纳米粒子催化鲁米诺体系的磁性微球检测赭曲霉毒素A化学发光适体传感器平台的构建
DOI：
10.1016/j.snb.2020.128375
复制
发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Xiang-Juan Zheng
中科院分区：
文献类型：
--
作者：
Xi-Luan Yan;Xin-Xin Xue;Jing Luo;Yu-Ting Jian;Li Tong;Xiang-Juan Zheng
In this paper, two kinds of chemiluminescent (CL) aptasensors for detecting Ochratoxin A (OTA) were constructed using Magnetic microsphere (MBs) as separated carrier. For the label-free aptasensor, in presence of the OTA, the OTA aptamer specially bound with it, and this affinity function was stronger than hybridization interaction between OTA aptamer and the paired sequence (capture probe), and resulted in the quantitative reduction of aptamers that bound to the capture probe on the MBs. And thus, according to the CL signals changes which came from the transient reaction between guanine (G) in the OTA aptamer sequence and phenylglyoxal (PG), the OTA could be rapidly detected. Meanwhile, another Au nanoparticle (Au NPs) -labeled aptasensor based on the above-mentioned mechanism of aptamer affinitive substitution was also prepared, analytical target OTA could be sensitively determined with the CL signals which were generated by Au NPs catalyzing luminol chemiluminescence. Under the optimal conditions, the detection limits of two OTA sensors were 0.041 ng/mL and 0.0067 ng/mL, respectively. What is more, these two simple, convenient and low-cost strategies afford high sensitivity, specificity and suitability for detection of OTA in real samples.
登录
查看更多内容
影响因子：
8.4
作者：
R. Soares;Diogo Ramadas;V. Chu;M. Aires-Barros;J. Conde;A. Viana;A. Cascalheira
通讯作者：
R. Soares;Diogo Ramadas;V. Chu;M. Aires-Barros;J. Conde;A. Viana;A. Cascalheira
影响因子：
8.4
作者：
Bueno Hernandez, Diana;Mishra, Rupesh K.;Marty, Jean Louis
通讯作者：
Marty, Jean Louis
影响因子：
8.4
作者：
Bu, Tong;Zhang, Meng;Wang, Li
通讯作者：
Wang, Li
基于链霉亲和素-生物素系统的免疫磁性纳米珠，用于高效、特异性分离单核细胞增生李斯特氏菌
影响因子：
6
作者：
Shan, Shan;Zhong, Ziqing;Liu, Daofeng
通讯作者：
Liu, Daofeng
影响因子：
6.1
作者：
J. Khang;Danial Kim;K. W. Chung;Ji Hoon Lee
通讯作者：
J. Khang;Danial Kim;K. W. Chung;Ji Hoon Lee