Quantitative validation of nicotine production in tea (Camellia sinensis L.).
Quantitative validation of nicotine production in tea (Camellia sinensis L.).
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DOI：
10.1371/journal.pone.0195422
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Morita A
中科院分区：
文献类型：
--
作者：
Ikka T;Yamashita H;Kurita I;Tanaka Y;Taniguchi F;Ogino A;Takeda K;Horie N;Hojo H;Nanjo F;Morita A
茶树（Camellia sinensis L.）通过液相色谱-串联质谱法对六个亚洲国家产茶区的茶叶样品进行了分析。所有样品均含有尼古丁（0.011-0.694 μg g−1干重）。在绿色茶、乌龙茶和红茶的生产过程中，尼古丁含量保持恒定，这意味着尼古丁在加工过程中对加热、干燥、酶促氧化和机械损伤是稳定的。品种Yabukita的花蕾和种子也含有尼古丁（0.030-0.041 μg g−1干重）。两个品种的比较表明，较高的尼古丁含量被发现在红茶品种Benifuki。水耕法矢北的所有植物部分都含有尼古丁（0.003-0.013 μg g−1干重）。在B5培养基中培养的茶细胞以及茶树幼苗的根和茎中含有与田间生长植物的新叶相似的尼古丁水平。虽然茶树中的内源性尼古丁水平极低，样品污染也不能排除，但这些水平超过了日本的最高可接受限度（0.01 μg g−1干重）。
Endogenous nicotine was confirmed to be present in tea plants (Camellia sinensis L.) by liquid chromatography-tandem mass spectrometry of tea samples from tea-producing regions in six Asian countries. All samples contained nicotine (0.011–0.694 μg g−1 dry weight). Nicotine contents remained constant during manufacturing of green, oolong and black teas, implying that nicotine is stable against heating, drying, enzymatic oxidation and mechanical damage during processing. Flower buds and seeds of cultivar Yabukita also contained nicotine (0.030–0.041 μg g−1 dry weight). A comparison of two cultivars revealed that higher nicotine contents were found in the black tea cultivar Benifuki. All plant parts of hydroponic Yabukita contained nicotine (0.003–0.013 μg g−1 dry weight). Tea cells cultured in B5 medium as well as roots and stems of tea seedlings contained nicotine levels similar to those of new leaves from field-grown plants. Although the levels of endogenous nicotine in tea plants are extremely low and sample contamination cannot be discounted, these levels exceed the maximum acceptable limit in Japan (0.01 μg g−1 dry weight).
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