Open simultaneous saccharification and fermentation of l-lactic acid by complete utilization of sweet sorghum stalk: a water-saving process.
Open simultaneous saccharification and fermentation of l-lactic acid by complete utilization of sweet sorghum stalk: a water-saving process.
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完全利用甜高粱秆开放式同步糖化发酵L-乳酸：节水工艺
DOI：
10.1039/d0ra09480c
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发表时间：
2021-01-25
期刊：
影响因子：
3.9
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Wang Y;Huo K;Gao L;Zhao G;Wang B;Liu J
以甜高粱秸秆为原料，通过开放式同步糖化发酵生产L-乳酸，实现了甜高粱汁和甜高粱渣的充分、高效利用。为了简化预处理工艺，降低水耗，采用了组合水解法，并利用NaOH预处理液（SL）作为部分中和剂。为了进一步提高产品效价，加入SSJ的酸水解物（SSJAH），并使用MgO作为中和剂。产物滴度为94 g L-1，产率为1.55 g L-1 h-1，产率达到98.31%。每kg甜高粱秸秆共产L-乳酸274.79 g，与碱预处理相比节水83.22%。本研究为木质纤维素底物生物合成l-乳酸提供了一条有效途径。
A complete and efficient utilization of sweet sorghum stalk including sweet sorghum juice (SSJ) and sweet sorghum bagasse (SSB) was achieved via the open simultaneous saccharification and fermentation (SSF) of l-lactic acid. To simplify the pretreatment process and reduce water consumption, a combined hydrolysis approach was applied and the NaOH-pretreated liquor (SL) was utilized as a partial neutralizing agent. In order to further enhance the product titer, the acid hydrolysate of SSJ (SSJAH) was fed, and MgO was used as a neutralizing agent. A product titer of 94 g L−1 was obtained with a productivity of 1.55 g L−1 h−1, and the yield reached 98.31%. Totally, 274.79 g l-lactic acid was produced from 1 kg sweet sorghum stalk, and 83.22% water was saved compared with the previous study based on alkali pretreatment of SSB. This study provides an effective process for l-lactic acid biosynthesis from lignocellulosic substrates.
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