AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis.
AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis.
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DOI：
10.1111/nph.12879
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发表时间：
2014-09
期刊：
影响因子：
--
通讯作者：
Coates JC
中科院分区：
文献类型：
--
作者：
Gibbs DJ;Voß U;Harding SA;Fannon J;Moody LA;Yamada E;Swarup K;Nibau C;Bassel GW;Choudhary A;Lavenus J;Bradshaw SJ;Stekel DJ;Bennett MJ;Coates JC
植物根系可塑性对于在不断变化的环境条件下生存至关重要。根结构的一个重要方面是侧根发育，这是一个受激素、环境和蛋白质信号通路调节的复杂过程。在这里，我们使用分子遗传学方法表明，MYB 转录因子 AtMYB93 是拟南芥侧根发育的新型负调节因子。我们将 AtMYB93 确定为侧根促进 ARABIDILLO 蛋白的相互作用伙伴。 Atmyb93突变体具有更快的侧根发育进程和增强的侧根密度，而AtMYB93过表达品系则表现出相反的表型。 AtMYB93 在覆盖早期侧根原基的内胚层细胞中强烈、特异且短暂地表达，并且在主根的基底分生组织中还受到生长素的诱导。此外，Atmyb93突变体侧根发育对生长素不敏感，表明AtMYB93是侧根发育过程中正常生长素反应所必需的。我们认为 AtMYB93 是一种新型生长素诱导的负反馈回路的一部分，该回路在侧根发育早期在选定的少数内胚层细胞中受到刺激，确保侧根仅在绝对需要时发育。推定的 AtMYB93 同源物在整个开花植物中均被检测到，并且代表了操纵不同作物物种根系的有希望的目标。
Plant root system plasticity is critical for survival in changing environmental conditions. One important aspect of root architecture is lateral root development, a complex process regulated by hormone, environmental and protein signalling pathways. Here we show, using molecular genetic approaches, that the MYB transcription factor AtMYB93 is a novel negative regulator of lateral root development in Arabidopsis. We identify AtMYB93 as an interaction partner of the lateral-root-promoting ARABIDILLO proteins. Atmyb93 mutants have faster lateral root developmental progression and enhanced lateral root densities, while AtMYB93-overexpressing lines display the opposite phenotype. AtMYB93 is expressed strongly, specifically and transiently in the endodermal cells overlying early lateral root primordia and is additionally induced by auxin in the basal meristem of the primary root. Furthermore, Atmyb93 mutant lateral root development is insensitive to auxin, indicating that AtMYB93 is required for normal auxin responses during lateral root development. We propose that AtMYB93 is part of a novel auxin-induced negative feedback loop stimulated in a select few endodermal cells early during lateral root development, ensuring that lateral roots only develop when absolutely required. Putative AtMYB93 homologues are detected throughout flowering plants and represent promising targets for manipulating root systems in diverse crop species.
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DOI：
10.1073/pnas.0507575103
发表时间：
2006-01-31
影响因子：
11.1
作者：
Coates, JC;Laplaze, L;Haseloff, J
通讯作者：
Haseloff, J
影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
7.2
作者：
Ivanchenko, Maria G.;Napsucialy-Mendivil, Selene;Dubrovsky, Joseph G.
通讯作者：
Dubrovsky, Joseph G.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
影响因子：
11.6
作者：
Duan, Lina;Dietrich, Daniela;Dinneny, Jose R.
通讯作者：
Dinneny, Jose R.