Non-Invasive Evaluation of Retinal Vascular Alterations in a Mouse Model of Optic Neuritis Using Laser Speckle Flowgraphy and Optical Coherence Tomography Angiography.
Non-Invasive Evaluation of Retinal Vascular Alterations in a Mouse Model of Optic Neuritis Using Laser Speckle Flowgraphy and Optical Coherence Tomography Angiography.
复制标题
使用激光散斑血流图和光学相干断层血管造影术对小鼠视神经炎模型中视网膜血管改变的非侵入性评价。
DOI：
10.3390/cells12232685
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发表时间：
2023-11-22
期刊：
影响因子：
6
通讯作者：
Liu, Hua
中科院分区：
文献类型：
--
作者：
Buscho, Seth E.;Xia, Fan;Shi, Shuizhen;Lin, Jonathan L.;Szczesny, Bartosz;Zhang, Wenbo;Motamedi, Massoud;Liu, Hua
关键词：
视神经炎是多发性硬化症（MS）的特征性特征，涉及视神经的炎症和视网膜神经节细胞（RGC）的变性。虽然先前的研究表明，视网膜血流改变发生在视神经炎，确切的位置，损害的程度，和潜在的机制仍然不清楚。在这项研究中，我们利用两种新兴的非侵入性成像技术，激光散斑血流图（LSFG）和光学相干断层扫描血管造影（OCTA），研究视网膜血管的变化，在小鼠模型MS，称为实验性自身免疫性脑脊髓炎（EAE）。我们将这些变化与白细胞停滞、RGC损伤和EAE的总体进展联系起来。LSFG成像显示EAE模型中视网膜血流速度进行性降低，视神经乳头附近血管阻力增加，表明眼部血流受损。OCTA成像显示EAE小鼠中深毛细血管丛的血管密度、连接数和总血管长度显著降低。此外，我们对白细胞停滞的分析显示，EAE小鼠视网膜血管中粘附的白细胞显著增加，这表明在EAE病理学的早期发展中发生了血管炎症。搜索者的改变先于或伴随视神经炎的特征性标志，如RGC丧失和视力下降。总的来说，我们的研究揭示了视网膜血管改变与视神经炎进展以及MS临床评分之间的复杂关系。它还强调了开发基于图像的生物标志物用于诊断和监测视神经炎以及MS的潜力，特别是在应对新兴治疗方面。
Optic neuritis, a characteristic feature of multiple sclerosis (MS), involves the inflammation of the optic nerve and the degeneration of retinal ganglion cells (RGCs). Although previous studies suggest that retinal blood flow alterations occur during optic neuritis, the precise location, the degree of impairment, and the underlying mechanisms remain unclear. In this study, we utilized two emerging non-invasive imaging techniques, laser speckle flowgraphy (LSFG) and optical coherence tomography angiography (OCTA), to investigate retinal vascular changes in a mouse model of MS, known as experimental autoimmune encephalomyelitis (EAE). We associated these changes with leukostasis, RGC injury, and the overall progression of EAE. LSFG imaging revealed a progressive reduction in retinal blood flow velocity and increased vascular resistance near the optic nerve head in the EAE model, indicating impaired ocular blood flow. OCTA imaging demonstrated significant decreases in vessel density, number of junctions, and total vessel length in the intermediate and deep capillary plexus of the EAE mice. Furthermore, our analysis of leukostasis revealed a significant increase in adherent leukocytes in the retinal vasculature of the EAE mice, suggesting the occurrence of vascular inflammation in the early development of EAE pathology. The abovechanges preceded or were accompanied by the characteristic hallmarks of optic neuritis, such as RGC loss and reduced visual acuity. Overall, our study sheds light on the intricate relationship between retinal vascular alterations and the progression of optic neuritis as well as MS clinical score. It also highlights the potential for the development of image-based biomarkers for the diagnosis and monitoring of optic neuritis as well as MS, particularly in response to emerging treatments.
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