Intraday variability of indicator and pathogenic viruses in 1-h and 24-h composite wastewater samples: Implications for wastewater-based epidemiology.
Intraday variability of indicator and pathogenic viruses in 1-h and 24-h composite wastewater samples: Implications for wastewater-based epidemiology.
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1小时和24小时复合废水样品中指示剂和致病病毒的日内变化：对废水流行病学的影响。
DOI：
10.1016/j.envres.2020.110531
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发表时间：
2021-03
影响因子：
8.3
通讯作者：
Korajkic A
中科院分区：
文献类型：
--
作者：
Ahmed W;Bivins A;Bertsch PM;Bibby K;Gyawali P;Sherchan SP;Simpson SL;Thomas KV;Verhagen R;Kitajima M;Mueller JF;Korajkic A
我们监测了指示病毒crasperiaage和辣椒温和斑驳病毒（PMMoV）和人类病原腺病毒（HAdV）的浓度从污水处理厂在布里斯班，澳大利亚在1小时和24小时的复合样品。在三天的采样中，24小时复合样本中crAsage基因拷贝数（GC）/mL的平均浓度没有显著差异（p = 0.72-0.92），而对于PMMoV GC/mL（p值范围：0.0002-0.0321）和HAdV GC/mL（p值范围：0.0028-0.0068）与其他两个采样日相比，在一个采样日观察到浓度的显著差异。对于所有三种病毒，在1小时复合样品中观察到的变化大于在24小时复合样品中观察到的变化。对于crAsphalage，在54.1%的1小时复合样品中，浓度低于24小时复合样品中观察到的浓度;而对于PMMoV和HAdV，浓度分别低于79.2%和70.9%的1小时复合样品，与相关的24小时复合样品相比。类似地，1小时复合样品中的crAsA浓度与24小时复合样品相比没有差异（p = 0.1082），而1小时复合样品中的PMMoV（p < 0.0001）和HAdV（p < 0.0001）浓度与24小时复合样品显著不同。这些结果表明，24小时的复合样品提供了更高的分析灵敏度和降低的变异性相比，1小时的复合样品监测废水时，特别是对致病性病毒与低感染率在一个社区。因此，对于基于废水的流行病学应用，24小时复合样本不太可能产生假阴性结果和错误的公共卫生信息。
We monitored the concentration of indicator viruses crAssphage and pepper mild mottle virus (PMMoV) and human pathogen adenovirus (HAdV) in influent from a wastewater treatment plant in Brisbane, Australia in 1-h and 24-h composite samples. Over three days of sampling, the mean concentration of crAssphage gene copies (GC)/mL in 24-h composite samples did not differ significantly (p = 0.72-0.92), while for PMMoV GC/mL (p value range: 0.0002-0.0321) and HAdV GC/mL (p value range: 0.0028-0.0068) significant differences in concentrations were observed on one day of sampling compared to the other two. For all three viruses, the variation observed in 1-h composite samples was greater than the variation observed in 24-h composite samples. For crAssphage, in 54.1% of 1-h composite samples, the concentration was less than that observed in 24-h composite samples; whereas for PMMoV and HAdV the concentration was less in 79.2 and 70.9% of 1-h composite samples, respectively, compared to the relevant 24-h composite samples. Similarly, the concentration of crAssphage in 1-h compared to 24-h composite samples did not differ (p = 0.1082) while the concentrations of PMMoV (p < 0.0001) and HAdV (p < 0.0001) in 1-h composite samples were significantly different from 24-h composite samples. These results suggest that 24-h composite samples offer increased analytical sensitivity and decreased variability compared to 1-h composite samples when monitoring wastewater, especially for pathogenic viruses with low infection rates within a community. Thus, for wastewater-based epidemiology applications, 24-h composite samples are less likely to produce false negative results and erroneous public health information.
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DOI：
10.1080/09603123.2013.769205
发表时间：
2013-12-01
影响因子：
3.2
作者：
Ganesh, Atheesha;Lin, Johnson
通讯作者：
Lin, Johnson
影响因子：
7.7
作者：
Lago, PM;Gary, HE;Cruz, RG
通讯作者：
Cruz, RG
影响因子：
2.7
作者：
Hamza, Hazem;Rizk, Neveen Magdy;Hamza, Ibrahim Ahmed
通讯作者：
Hamza, Ibrahim Ahmed
影响因子：
12.8
作者：
Madoux-Humery, Anne-Sophie;Dorner, Sarah;Prevost, Michele
通讯作者：
Prevost, Michele
影响因子：
9.8
作者：
Haramoto, Eiji;Malla, Bikash;Kitajima, Masaaki
通讯作者：
Kitajima, Masaaki