Comparative ultrastructure of leukotoxic and non-leukotoxic strains of Actinobacillus actinomycetemcomitans.
Comparative ultrastructure of leukotoxic and non-leukotoxic strains of Actinobacillus actinomycetemcomitans.
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放线放线杆菌伴白细胞毒性和非白细胞毒性菌株的超微结构比较。
DOI：
10.1111/j.1600-0765.1981.tb00989.x
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发表时间：
1981
影响因子：
3.5
通讯作者：
Hammond,BF
中科院分区：
文献类型：
--
作者：
Lai,CH;Listgarten,MA;Hammond,BF
14株放线共生放线杆菌（Actinobacillus actinomycetemcomitans，A.放线菌属）已通过透射电子显微镜检查。这些细胞在琼脂培养基上以球杆菌形式生长，其细胞周边在超微结构上与其他革兰氏阴性菌种相容。然而，在标准处理后，细胞膜和肽聚糖层不容易可视化。特别有趣的是，在已知具有白细胞毒性活性的伴放线菌菌株中发现了大量细胞外膜囊泡，而非白细胞毒性菌株则表现出很少或没有细胞外囊泡。无论细胞在血琼脂平板上还是在巯基乙酸盐肉汤中生长，都观察到类似的结果。在兔中获得抗白细胞毒性菌株Y4的全细胞的抗血清。所得抗血清成功地标记与4个单独的白细胞毒性菌株相关的囊泡。在用非白细胞毒性菌株ATCC 29523吸收抗血清后，标签完全保留在囊泡上。非白细胞毒性菌株在未吸收的Y4抗血清中显示出一些斑点状表面标记，在ATCC菌株29523吸收后未显示标记。这些观察结果表明，细胞外膜囊泡与白细胞毒活性，并可能作为一个有用的来源，白细胞毒因子。这些结论得到加强，通过观察细胞外囊泡在琼脂上生长的菌株的浓度和它们的相对白细胞毒性之间的强相关性，如其他研究者所报告的。
Fourteen strains ofActinobacillus actinomycetemcomitans (A. actinomycetemcomitans)have been examined by transmission electron microscopy. The cells, which grow as coccobacillary forms on agar medium, have a cell periphery ultrastructurally compatible with that of other Gram‐negative species. However, the cell membrane and peptidoglycan layer are not readily visualized following standard processing. Of particular interest was the finding of numerous extracellular membranous vesicles inA.actinomycetemcomitansstrains known to have leukotoxic activity, while non‐leukotoxic strains demonstrated few or no extracellular vesicles. Similar findings were noted whether cells were grown on blood agar plates or in thioglycollate broth. Antisera were obtained in rabbits against whole cells of leukotoxic strain Y4. The resulting antisera successfully labeled vesicles associated with 4 separate leukotoxic strains. The label was completely retained on the vesicles after absorption of the antisera with non‐leukotoxic strain ATCC 29523. Non‐leukotoxic strains showed some patchy surface labeling with non‐absorbed antiserum to Y4 and no labeling after absorption with ATCC strain 29523. These observations suggest that extracellular membranous vesicles are associated with leukotoxic activity and may serve as a useful source of leukotoxic factor. These conclusions are strengthened by the observation of a strong correlation between the concentration of extracellular vesicles in strains grown on agar and their relative leukotoxicity as reported by other investigators.
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