Measurement of trimethylamine-N-oxide by stable isotope dilution liquid chromatography tandem mass spectrometry.
Measurement of trimethylamine-N-oxide by stable isotope dilution liquid chromatography tandem mass spectrometry.
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DOI：
10.1016/j.ab.2014.03.016
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发表时间：
2014-06-15
影响因子：
2.9
通讯作者：
Hazen SL
中科院分区：
文献类型：
--
作者：
Wang Z;Levison BS;Hazen JE;Donahue L;Li XM;Hazen SL
血液中的三甲基胺-N-氧化物（TMAO）水平可预测未来主要不良心脏事件的风险，包括心肌梗死、中风和死亡。因此，快速测定循环TMAO浓度具有临床意义。在这里，我们报告了一种通过稳定同位素稀释液相色谱串联质谱（LC/MS/MS）测量生物基质中TMAO的方法，其定量下限和上限分别为0.05和>200 µM。加标和回收率研究表明，低（0.5 µM）、中（5 µM）和高（100 µM）水平下的准确度分别为98.2%、97.3%和101.6%。另外的测定性能度量包括在TMAO水平的范围内分别< 6.4%和<9.9%的日内和日间方差系数。稳定性研究表明，血浆中的TMAO在−80 °C下储存5年和多次冻融循环期间都是稳定的。在表面健康受试者（n=349）中进行的空腹血浆正常范围研究显示，范围为0.73 - 126 µM，中位（四分位距）水平为3.45（2.25-5.79）µM，且数值随年龄增加而增加。LC/MS/MS为基础的分析报告应该是有价值的进一步研究评估TMAO作为一种风险标志物，并检查饮食，药理学和环境因素对TMAO水平的影响。
Trimethylamine-N-oxide (TMAO) levels in blood predict future risk for major adverse cardiac events including myocardial infarction, stroke and death. Thus, the rapid determination of circulating TMAO concentration is of clinical interest. Here we report a method to measure TMAO in biological matrices by stable isotope dilution liquid chromatography tandem mass spectrometry (LC/MS/MS) with lower and upper limits of quantification of 0.05 and >200 µM, respectively. Spike and recovery studies demonstrate an accuracy at low (0.5 µM), mid (5 µM) and high (100 µM) levels of 98.2%, 97.3% and 101.6%, respectively. Additional assay performance metrics include intra-day and inter-day coefficients of variance of < 6.4% and < 9.9%, respectively, across the range of TMAO levels. Stability studies reveal TMAO in plasma is stable both during storage at −80 °C for 5 years and to multiple freeze thaw cycles. Fasting plasma normal range studies among apparently healthy subjects (n=349) shows a range of 0.73 – 126 µM, median (interquartile range) levels of 3.45 (2.25–5.79) µM, and increasing values with age. The LC/MS/MS based assay reported should be of value for further studies evaluating TMAO as a risk marker and for examining the effect of dietary, pharmacologic and environmental factors on TMAO levels.
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DOI：
10.1161/atvbaha.109.185645
发表时间：
2009-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Wang Z;Tang WH;Cho L;Brennan DM;Hazen SL
通讯作者：
Hazen SL
DOI：
10.1056/nejmoa1109400
发表时间：
2013-04-25
期刊：
The New England journal of medicine
影响因子：
--
作者：
Tang WH;Wang Z;Levison BS;Koeth RA;Britt EB;Fu X;Wu Y;Hazen SL
通讯作者：
Hazen SL
影响因子：
4.1
作者：
Erupe, Mark E.;Liberman-Martin, Allegra;Purvis-Roberts, Kathleen L.
通讯作者：
Purvis-Roberts, Kathleen L.
影响因子：
2.9
作者：
Mamer, OA;Choinière, L;Treacy, EP
通讯作者：
Treacy, EP
影响因子：
3.2
作者：
STENBERG, E;STYRVOLD, OB;STROM, AR
通讯作者：
STROM, AR