Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.

Super-resolution Microscopy with Single Molecules in Biology and Beyond-Essentials, Current Trends, and Future Challenges.
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DOI:
10.1021/jacs.0c08178
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发表时间:
2020-10-21
影响因子:
15
通讯作者:
Moerner WE
Moerner WE
中科院分区:
化学1区
文献类型:
--
作者:
Möckl L;Moerner WE

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在过去的二十年里,单分子超分辨率显微镜已经从一种专门的技术发展成为纳米尺度上最通用和最强大的成像方法之一。从这个角度来看,我们提供了该领域历史发展的简要概述,基本概念,获得最大定量信息所需的方法,以及当前的艺术状态。然后,我们将讨论新兴的观点和需要创新和进一步改进的领域。尽管取得了巨大的进步,但单分子超分辨率显微镜的全部潜力尚未实现,这将由我们前面的研究实现。
Single-molecule super-resolution microscopy has developed from a specialized technique into one of the most versatile and powerful imaging methods of the nanoscale over the past two decades. In this perspective, we provide a brief overview of the historical development of the field, the fundamental concepts, the methodology required to obtain maximum quantitative information, and the current state of the art. Then, we will discuss emerging perspectives and areas where innovation and further improvement are needed. Despite the tremendous progress, the full potential of single-molecule super-resolution microscopy is yet to be realized, which will be enabled by the research ahead of us.
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