OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice
OsYSL16 is Required for Preferential Cu Distribution to Floral Organs in Rice
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OsYSL16 是铜优先分配到水稻花器官所必需的
DOI：
10.1093/pcp/pcy124
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发表时间：
2018-06
期刊：
影响因子：
--
通讯作者：
Luqing Zheng
中科院分区：
文献类型：
--
作者：
Chang Zhang;Wenhui Lu;Yang Yang;Zhenguo Shen;Jian Feng Ma;Luqing Zheng
铜缺乏导致许多植物的育性低下，但铜在花器官中分布的分子机制尚不清楚。在此，我们发现编码烟胺转运蛋白(Cu-NA)的YSL家族成员YSL16在小穗轴中高表达，而在水稻的颖片和外膜中表达较少。对转OsYSL16启动子-GUS的转基因水稻进行β-GUS染色，结果表明，OsYSL16主要在小穗轴的维管束和颖片中表达。OsYSL16基因的敲除导致铜在雄蕊中的分配减少，但在内颖和外膜中的分配增加。短期(24小时)~(65)Cu标记实验证实，突变体的内颖和外颖中的铜浓度增加。此外，我们还发现，在osysl16突变体中，暴露于无铜溶液后，铜从颖片和外膜中的再分配受到了损害。Osysl16突变体表现出较低的花粉萌发率，但这一点可以通过在培养基中添加铜来挽救。我们的结果表明，水稻小穗轴维管束中表达的OsYSL16基因对铜优先分配到雄蕊中起重要作用，而在缺铜条件下，水稻颖壳和外颖维管束中表达的OsYSL16参与了铜的再分配。
Deficiency of copper (Cu) causes low fertility in many plant species, but the molecular mechanisms underlying distribution of Cu to the floral organs are poorly understood. Here, we found that a member of yellow-stripe like (YSL) family, YSL16 encoding the Cu-nicotianamine (Cu-NA) transporter, was highly expressed in the rachilla, with less expression in the palea and lemma of rice (Oryza sativa). β-Glucuronidase (GUS) staining of transgenic rice carrying the OsYSL16 promoter-GUS showed that OsYSL16 was mainly expressed in vascular bundles of the rachilla as well as the palea and lemma. Knockout of OsYSL16 resulted in decreased Cu distribution to the stamens, but increased distribution to the palea and lemma. A short-term (24 h) 65Cu labeling experiment confirmed increased Cu concentration of palea and lemma in the mutant. Furthermore, we found that redistribution of Cu from the palea and lemma was impaired in the osysl16 mutant after exposure to Cu-free solution. The osysl16 mutant showed low pollen germination, but this was rescued by addition of Cu in the medium. Our results indicate that OsYSL16 expressed in the vascular bundles of the rachilla is important for preferential distribution of Cu to the stamens, while OsYSL16 in vascular bundles of the palea and lemma is involved in Cu redistribution under Cu-limited conditions in rice.
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