Chitosan-Modified Graphene Electrodes for DNA Mutation Analysis.
Chitosan-Modified Graphene Electrodes for DNA Mutation Analysis.
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DOI：
10.1016/j.jelechem.2012.09.026
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发表时间：
2012-10-15
期刊：
影响因子：
--
通讯作者：
Li CZ
中科院分区：
文献类型：
--
作者：
Alwarappan S;Cissell K;Dixit S;Mohapatra S;Li CZ
石墨烯具有显著的电化学性质，使其成为构建生物传感器的理想材料，但尚未探索用于DNA生物传感。在此，我们报告了一个壳聚糖修饰的石墨烯平台，用于电化学检测DNA序列的变化。为了这个目的，石墨烯化学合成和拉曼光谱和透射电子显微镜，其特征在于，共价修饰带正电荷的壳聚糖，以促进固定化的单链DNA“捕获”寡核苷酸。通过红外光谱证实了壳聚糖与石墨烯的共价连接，然后将捕获DNA固定在壳聚糖修饰的石墨烯电极上。然后，将靶DNA（互补或错配的“突变体”DNA）施加到电极上，并进行循环伏安法。伏安实验的结果表明，固定有ssDNA+互补DNA的壳聚糖修饰石墨烯电极表现出比固定有非互补突变DNA的壳聚糖修饰石墨烯电极显著更高的氧化还原峰电流。总之，这些结果表明，壳聚糖-石墨烯平台提供了对错配DNA的快速、稳定和灵敏的检测，并有可能用于与疾病相关的特定DNA突变的即时诊断测试。
Graphene has remarkable electrochemical properties that make it an ideal material for constructing biosensors,however it has not been explored for DNA biosensing. Herein, we report on a chitosan-modified graphene platform for the electrochemical detection of changes in DNA sequences. For this purpose, graphene synthesized chemically and characterized by Raman spectroscopy and Transmission electron microscopy, was covalently modified with positively charged chitosan to facilitate the immobilization of a single-stranded DNA `capture' oligonucleotide. The covalent attachment of chitosan to graphene was confirmed by FT-IR spectroscopy and then the capture DNA was immobilized on to the chitosan modified graphene electrode. Then, the target DNA (complementary or mismatched `mutant' DNA) was applied to the electrode and cyclic voltammetry was performed. The results of the voltammetric experiments indicate that the chitosan modified graphene electrodes immobilized with ssDNA+complementary DNA exhibit a significantly higher magnitude of redox peak current than the chitosan modified graphene electrodes immobilized with the non-complementary mutant DNAs. Together, these results demonstrate that the chitosan-graphene platform provides a rapid, stable and sensitive detection of mismatched DNA and has the potential to be used for point-of-care diagnostic tests for specific DNA mutations associated with disease conditions.
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