Analysis of hepatitis C virus RNA dimerization and core-RNA interactions.
Analysis of hepatitis C virus RNA dimerization and core-RNA interactions.
复制标题
丙型肝炎病毒 RNA 二聚化和核心 RNA 相互作用分析。
DOI：
10.1093/nar/gkl240
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发表时间：
2006
影响因子：
14.9
通讯作者：
Darlix JL
中科院分区：
文献类型：
--
作者：
Ivanyi-Nagy R;Kanevsky I;Gabus C;Lavergne JP;Ficheux D;Penin F;Fossé P;Darlix JL
The core protein of hepatitis C virus (HCV) has been shown previously to act as a potent nucleic acid chaperone in vitro, promoting the dimerization of the 3′-untranslated region (3′-UTR) of the HCV genomic RNA, a process probably mediated by a small, highly conserved palindromic RNA motif, named DLS (dimer linkage sequence) [G. Cristofari, R. Ivanyi-Nagy, C. Gabus, S. Boulant, J. P. Lavergne, F. Penin and J. L. Darlix (2004) Nucleic Acids Res., 32, 2623–2631]. To investigate in depth HCV RNA dimerization, we generated a series of point mutations in the DLS region. We find that both the plus-strand 3′-UTR and the complementary minus-strand RNA can dimerize in the presence of core protein, while mutations in the DLS (among them a single point mutation that abolished RNA replication in a HCV subgenomic replicon system) completely abrogate dimerization. Structural probing of plus- and minus-strand RNAs, in their monomeric and dimeric forms, indicate that the DLS is the major if not the sole determinant of UTR RNA dimerization. Furthermore, the N-terminal basic amino acid clusters of core protein were found to be sufficient to induce dimerization, suggesting that they retain full RNA chaperone activity. These findings may have important consequences for understanding the HCV replicative cycle and the genetic variability of the virus.
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影响因子：
12.7
作者：
Bukh, J;Apgar, CL;Purcell, RH
通讯作者：
Purcell, RH
影响因子：
5.4
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Friebe, P;Bartenschlager, R
通讯作者：
Bartenschlager, R
影响因子：
5.4
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Boulant, S;Vanbelle, C;Lavergne, JP
通讯作者：
Lavergne, JP
影响因子：
5.4
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Kalinina, O;Norder, H;Magnius, LO
通讯作者：
Magnius, LO
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3.8
作者：
Kalinina, O;Norder, H;Magnius, LO
通讯作者：
Magnius, LO