Aptamer-modified magnetic beads in affinity separation of proteins.
Aptamer-modified magnetic beads in affinity separation of proteins.
复制标题
适配体修饰磁珠在蛋白质亲和分离中的应用
DOI:
10.1007/978-1-4939-2447-9_7
复制
发表时间:
2015
影响因子:
--
通讯作者:
Johanna-Gabriela Walter
中科院分区:
文献类型:
--
作者:
Guohong Zhu;Johanna-Gabriela Walter
Aptamers are valuable alternative ligands for affinity separations. Here, we describe the aptamer-based affinity separation of His-tagged proteins using an aptamer directed against the His-tag. The immobilization of the aptamer to magnetic beads is described as well as the aptamer-based purification and proper methods for the characterization of the process. Moreover, indications for the transfer of the process to other aptamers are given.
影响因子:
2.7
作者:
J. Walter;F. Stahl;T. Scheper
通讯作者:
J. Walter;F. Stahl;T. Scheper
影响因子:
2.1
作者:
G. Zhu;M. Lübbecke;J. Walter;F. Stahl;T. Scheper
通讯作者:
T. Scheper
影响因子:
56.9
作者:
TUERK, C;GOLD, L
通讯作者:
GOLD, L