Cloning and characterization of a putative TAC1 ortholog associated with leaf angle in maize (Zea mays L.).
Cloning and characterization of a putative TAC1 ortholog associated with leaf angle in maize (Zea mays L.).
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DOI：
10.1371/journal.pone.0020621
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Ku L;Wei X;Zhang S;Zhang J;Guo S;Chen Y
在高密度栽培条件下，通过改变植株构型，提高光合效率，减少避荫反应，对进一步提高作物产量具有重要意义。玉米叶片角度控制基因的鉴定有助于深入了解叶片发育的分子机制，实现理想的植株构型以提高产量。采用比较基因组学方法进行基因克隆，并进行实时荧光定量PCR（RT-PCR）分析基因表达。通过序列不相似性分析和功能性切割扩增多态性（CAP）QTL定位验证基因功能。叶角受一个主效数量性状基因座ZmTAC 1控制。叶片角度控制1）。ZmTAC 1有4个外显子，编码263个氨基酸的蛋白质，其结构域与水稻OsTAC 1蛋白的结构域相同。利用CAP标记和豫82与沈137杂交的F2：3家系分析，发现ZmTAC 1位于qLA 2区域。实时荧光定量PCR分析表明，ZmTAC 1在叶鞘枕中表达量最高，在叶和茎顶端分生组织中表达量较少，在根中表达量最低。紧密自交系豫82（“CTCC”）和扩展自交系沈137（“CCCC”）之间5′-非翻译区（UTR）的核苷酸差异影响ZmTAC 1的表达水平，从而进一步控制叶角的大小。序列验证结果表明，ZmTAC 1基因在13个紧凑型自交系和18个扩展型自交系中分别含有“CTCC”和“CCCC”基因，表明ZmTAC 1基因在玉米株型改良中具有广泛的应用价值。
Modifying plant architecture to increase photosynthesis efficiency and reduce shade avoidance response is very important for further yield improvement when crops are grown in high density. Identification of alleles controlling leaf angle in maize is needed to provide insight into molecular mechanism of leaf development and achieving ideal plant architecture to improve grain yield. The gene cloning was done by using comparative genomics, and then performing real-time polymerase chain reaction (RT-PCR) analysis to assay gene expression. The gene function was validated by sequence dissimilarity analysis and QTL mapping using a functional cleaved amplified polymorphism (CAP). The leaf angle is controlled by a major quantitative trait locus, ZmTAC1 (Zea mays L. Leaf Angle Control 1). ZmTAC1 has 4 exons encoding a protein with 263 amino acids, and its domains are the same as those of the rice OsTAC1 protein. ZmTAC1 was found to be located in the region of qLA2 by using the CAP marker and the F2:3 families from the cross between Yu82 and Shen137. Real-time PCR analysis revealed ZmTAC1 expression was the highest in the leaf-sheath pulvinus, less in the leaf and shoot apical meristem, and the lowest in the root. A nucleotide difference in the 5′-untranslated region (UTR) between the compact inbred line Yu82 (“CTCC”) and the expanded inbred line Shen137 (“CCCC”) influences the expression level of ZmTAC1, further controlling the size of the leaf angle. Sequence verification of the change in the 5′-UTR revealed ZmTAC1 with “CTCC” was present in 13 compact inbred lines and ZmTAC1 with “CCCC” was present in 18 expanded inbred lines, indicating ZmTAC1 had been extensively utilized in breeding with regard to the improvement of the maize plant architecture.
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影响因子：
4.2
作者：
Sanmiguel, P;Bennetzen, JL
通讯作者：
Bennetzen, JL
影响因子：
--
作者：
Wu, Q;Mao, XH
通讯作者：
Mao, XH
控制水稻驯化过程中从平伏生长到直立生长的关键转变
影响因子：
30.8
作者：
Tan, Lubin;Li, Xianran;Sun, Chuanqing
通讯作者：
Sun, Chuanqing
影响因子：
2.3
作者：
Mickelson, SM;Stuber, CS;Kaeppler, SM
通讯作者：
Kaeppler, SM
影响因子：
46.9
作者：
Sakamoto, T;Morinaka, Y;Matsuoka, M
通讯作者：
Matsuoka, M