Curvature gradient drives polarized tissue flow in the Drosophila embryo.
Curvature gradient drives polarized tissue flow in the Drosophila embryo.
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DOI：
10.1073/pnas.2214205120
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发表时间：
2023-02-07
影响因子：
11.1
通讯作者：
Lecuit, Thomas
中科院分区：
文献类型：
--
作者：
Gehrels, Emily W.;Chakrabortty, Bandan;Perrin, Marc-Eric;Merkel, Matthias;Lecuit, Thomas
关于胚胎的遗传预模式如何定义形态发生的初始指令，我们知道得很多，但这些指令如何在特定的机械和几何环境中部署，我们还不知道。在我们的手稿中，我们使用果蝇胚胎来探索遗传学、力学和几何学如何相互作用来驱动极化（定向）组织流动。通过实验和建模方法相结合，我们表明，以前提出的机制不能解释果蝇形态发生的早期阶段观察到的组织流。相反，我们揭示了一种机制，即极化流产生肌球蛋白驱动的组织收缩和鸡蛋的形状所施加的组织的曲率之间的相互作用。形态发生过程中的组织流动通常由细胞皮质的局部收缩驱动，这是由肌动球蛋白收缩性的激活引起的。由于组织粘性，这可能导致长距离流动。然而，在缺乏细胞内在的极化力或组织外部力的极性的情况下，这些流动必须是对称的，并且以收缩区域为中心。极化的组织流动先前已经被证明是由这种收缩性流动与外部结构的摩擦或粘附增加的点的耦合引起的。然而，我们的实验和建模表明，在早期果蝇形态发生的极化组织流的发生独立的粘附，而是由顶端肌动球蛋白收缩性的几何耦合驱动的组织曲率。特别地，极化流的开始是由顶端肌球蛋白激活的位置与胚胎后极处的峰值曲率的位置之间的不匹配驱动的。我们的工作演示了遗传和几何信息从母亲继承如何相互作用，以创建在胚胎形态发生极化流。
Much is known about how genetic prepatterning of the embryo defines the initial instructions for morphogenesis, but how these instructions are deployed in a specific mechanical and geometrical environment is unknown. In our manuscript, we use Drosophila embryos to explore how genetics, mechanics, and geometry interact to drive polarized (directional) tissue flow. Through a combination of experimental and modeling approaches, we show that previously proposed mechanisms cannot account for the tissue flows observed during the early stages of Drosophila morphogenesis. Instead, we reveal a mechanism whereby polarized flows arise from the interaction between myosin-driven tissue contraction and the curvature of the tissue imposed by the shape of the egg. Tissue flow during morphogenesis is commonly driven by local constriction of cell cortices, which is caused by the activation of actomyosin contractility. This can lead to long-range flows due to tissue viscosity. However, in the absence of cell-intrinsic polarized forces or polarity in forces external to the tissue, these flows must be symmetric and centered around the region of contraction. Polarized tissue flows have been previously demonstrated to arise from the coupling of such contractile flows to points of increased friction or adhesion to external structures. However, we show with experiments and modeling that the onset of polarized tissue flow in early Drosophila morphogenesis occurs independent of adhesion and is instead driven by a geometric coupling of apical actomyosin contractility to tissue curvature. Particularly, the onset of polarized flow is driven by a mismatch between the position of apical myosin activation and the position of peak curvature at the posterior pole of the embryo. Our work demonstrates how genetic and geometric information inherited from the mother interact to create polarized flow during embryo morphogenesis.
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DOI：
10.1038/s41578-021-00279-y
发表时间：
2021
期刊：
Nature reviews. Materials
影响因子：
--
作者：
Hofer M;Lutolf MP
通讯作者：
Lutolf MP
影响因子：
64.8
作者：
Martin, Adam C.;Kaschube, Matthias;Wieschaus, Eric F.
通讯作者：
Wieschaus, Eric F.
影响因子：
21.3
作者：
Kerridge, Stephen;Munjal, Akankshi;Lecuit, Thomas
通讯作者：
Lecuit, Thomas
影响因子：
7.7
作者：
Guirao, Boris;Rigaud, Stephane U.;Bellaiche, Yohanns
通讯作者：
Bellaiche, Yohanns
影响因子：
4.6
作者：
He, Bing;Martin, Adam;Wieschaus, Eric
通讯作者：
Wieschaus, Eric