Reactive human bile ductules express parathyroid hormone‐related peptide
Reactive human bile ductules express parathyroid hormone‐related peptide
复制标题
反应性人胆管表达甲状旁腺激素相关肽
作者：
T. Roskams;R. Campos;R. Campos;D. Drucker;D. Drucker;V. Desmet
各种胆汁淤积性肝病以及次大面积坏死后的再生都伴随着胆小管数量的显著增加。这些反应性胆管被认为起源于原有胆管或胆管相关兼性干细胞的增殖，或源于肝细胞的管状化生。最近，我们发现反应性胆管具有神经内分泌特征，并推测神经内分泌颗粒中产生的S物质可能通过自分泌或旁分泌途径对其生长和/或分化起作用。甲状旁腺激素相关肽已被证明是由一个生长因子调节的基因编码的，该基因可能在细胞的生长和分化中发挥作用。我们对3例正常肝、11例胆汁淤积性肝病、6例局灶性结节增生和3例再生肝进行了免疫组织化学研究。在再生肝、原发性胆汁性肝硬变、原发性硬化性胆管炎和部分或间歇性梗阻中，大多数表达神经内分泌标志物的反应性导管细胞也表达甲状旁腺激素相关肽。在局灶性结节增生症中，少量胆管细胞表达该肽。这些发现表明甲状旁腺激素相关肽定位于胆管细胞，并可能提示该激素在人反应性胆管的生长和/或分化中起作用。
Various cholestatic liver diseases as well as regeneration after submassive necrosis are accompanied by a striking increase in the number of bile ductules. These reactive bile ductules are thought to arise either from proliferation of pre‐existing bile ductules or bile ductule‐related facultative stem cells, or from ductular metaplasia of hepatocytes. Recently, we found that reactive bile ductules display neuro‐endocrine features, and speculated that the substance(s), produced in the neuro‐endocrine granules, might play a role in their growth and/or differentiation through an autocrine or paracrine pathway. Parathyroid hormone‐related peptide has been shown to be encoded by a growth factor‐regulated gene that may play a role in cell growth and differentiation. We studied the immunohistochemical expression of this peptide in human liver, including three normal biopsies, 11 cases of cholestatic liver disease, six cases of focal nodular hyperplasia and three cases of regenerating liver. In regenerating liver, primary biliary cirrhosis, primary sclerosing cholangitis and partial or intermittent obstruction, the majority of reactive ductular cells expressing neuro‐endocrine markers also expressed parathyroid hormone‐related peptide. In focal nodular hyperplasia, a smaller number of bile ductular cells expressed the peptide. These findings suggest that parathyroid hormone‐related peptide is localized in bile ductular cells and may indicate a role for this hormone in the growth and/or differentiation of human reactive bile ductules.
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DOI：
10.1016/s0021-9258(18)48258-0
发表时间：
1987-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Ignotz;T. Endo;J. Massagu
通讯作者：
R. Ignotz;T. Endo;J. Massagu
胚胎癌细胞分化过程中甲状旁腺激素样蛋白及其受体的表达。
DOI：
10.1210/mend-4-4-638
发表时间：
1990
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Chan,SD;Strewler,GJ;King,KL;Nissenson,RA
通讯作者：
Nissenson,RA
肝脏生长的调节：原癌基因和转化生长因子。
DOI：
--
发表时间：
1989
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Fausto,N;Mead,JE
通讯作者：
Mead,JE
影响因子：
11.2
作者：
R. Campos;S. Asa;D. Drucker
通讯作者：
R. Campos;S. Asa;D. Drucker
放线菌酮对甲状旁腺激素相关肽基因表达的调节。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ikeda,K;Lu,C;Weir,EC;Mangin,M;Broadus,AE
通讯作者：
Broadus,AE