A simple scoring of beam walking performance after spinal cord injury in mice.
A simple scoring of beam walking performance after spinal cord injury in mice.
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DOI：
10.1371/journal.pone.0272233
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
需要使用简单且可重复的脊髓损伤（SCI）小鼠模型测试来精确评估运动功能。SCI小鼠的地上行走已经通过Basso小鼠运动量表（BMS）测试。相比之下，只有少数作品量化了SCI小鼠在窄梁上的行走表现，这是一项不同的任务。在这里，我们建立了一个新的评分系统，使用单梁步行装置SCI小鼠。评分系统使用二元判断的价值观，如保留，前进和达到目标的一个横梁上的评级。此外，当小鼠有效地使用后肢在平衡木上运动时，给予SCI小鼠高分。两名观察者的后肢位置评分一致率很高。脊髓损伤后即刻，小鼠在横梁上的总得分最低，然后得分逐渐增加，与BMS得分的时间过程相似。此外，总评分反映了两个品系小鼠SCI严重程度的分级。平衡木行走评分被证明与BMS评分密切相关，表明SCI小鼠地上行走和平衡木行走之间的表现部分相关。总的来说，新的评分系统提供了一个机会，很容易评估运动表现的小鼠与SCI。
Precise evaluation of motor functions using simple and reproducible tests for mouse models of spinal cord injury (SCI) are required. Overground walking of SCI mice has been tested by Basso Mouse Scale for locomotion (BMS). In contrast, only a few works quantify walking performances of SCI mice on narrow beams, a different task. Here, we established a novel scoring system using a single beam walking apparatus for SCI mice. The scoring system uses binary judgments of values such as retention, moving forward and reaching the goal on a beam for rating. In addition, high score was given to SCI mouse when the mouse efficiently used hindlimbs for locomotion on the beam. A high rate of concordance of the score derived from positions of hindlimbs between two observers was obtained. Mice displayed the lowest total score on the beam immediately after the SCI, then the score gradually increased like time course of BMS score. Furthermore, the total scores reflected gradation of severity of SCI in 2 strains of mice. The beam walking score proved to be strongly correlated with that of BMS score, indicating that performances between overground walking and beam walking are partly correlated in SCI mice. Collectively, the novel scoring system offers an opportunity to easily evaluate motor performances of mice with SCI.
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影响因子：
7.5
作者：
Amemori T;Ruzicka J;Romanyuk N;Jhanwar-Uniyal M;Sykova E;Jendelova P
通讯作者：
Jendelova P
影响因子：
2.8
作者：
Li, JS;Oskouian, RJ;Linden, J
通讯作者：
Linden, J
影响因子：
4.2
作者：
Fiore, C;Inman, DM;Compagnone, NA
通讯作者：
Compagnone, NA
DOI：
10.5662/wjm.v7.i2.68
发表时间：
2017-06-26
期刊：
World journal of methodology
影响因子：
--
作者：
Shroff, Geeta;Barthakur, Jitendra Kumar
通讯作者：
Barthakur, Jitendra Kumar
影响因子：
7.4
作者：
Cheng, Peng;Kuang, Fang;Ju, Gong
通讯作者：
Ju, Gong