Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro.
Aligned fibrous PVDF-TrFE scaffolds with Schwann cells support neurite extension and myelination in vitro.
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DOI：
10.1088/1741-2552/aac77f
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发表时间：
2018-10
影响因子：
4
通讯作者：
Arinzeh TL
中科院分区：
文献类型：
--
作者：
Wu S;Chen MS;Maurel P;Lee YS;Bunge MB;Arinzeh TL
聚偏二氟乙烯-三氟乙烯（PVDF-TrFE）是一种压电生物相容性聚合物，有望作为与许旺细胞（SC）结合的脊髓修复支架。压电材料可以响应于机械变形而产生电活动，这可能潜在地刺激脊髓轴突再生。我们在这项研究中的目标是研究PVDF-TrFE支架组成的对齐的纤维在支持SC的生长和SC支持的神经突起的延伸和髓鞘形成在体外。采用静电纺丝技术制备了PVDF-TrFE取向纤维。将SC和背根神经节（DRG）外植体共培养以评估PVDF-TrFE支架上SC支持的神经突延伸和髓鞘形成。PVDF-TrFE支架支持SC生长和神经突延伸，这通过用Matrigel涂覆支架而进一步增强。SC被定向并且神经突沿对齐的纤维的长度沿着延伸。与没有SC的支架相比，在PVDF-TrFE支架上与DRG共培养的SC促进更长的神经突延伸。除了促进神经突延伸之外，SC还在PVDF-TrFE支架上的DRG神经突周围形成髓鞘。该研究证明了含有对齐纤维的PVDF-TrFE支架支持SC-神经突延伸和髓鞘形成。结论：干细胞与PVDF-TrFE支架的联合应用有望成为脊髓损伤修复的组织工程策略。
Polyvinylidene fluoride-trifluoroethylene (PVDF-TrFE), which is a piezoelectric, biocompatible polymer, holds promise as a scaffold in combination with Schwann cells (SCs) for spinal cord repair. Piezoelectric materials can generate electrical activity in response to mechanical deformation, which could potentially stimulate spinal cord axon regeneration. Our goal in this study was to investigate PVDF-TrFE scaffolds consisting of aligned fibers in supporting SC growth and SC-supported neurite extension and myelination in vitro. Aligned fibers of PVDF-TrFE were fabricated using the electrospinning technique. SCs and Dorsal Root Ganglion (DRG) explants were co-cultured to evaluate SC-supported neurite extension and myelination on PVDF-TrFE scaffolds. PVDF-TrFE scaffolds supported SC growth and neurite extension, which was further enhanced by coating the scaffolds with Matrigel. SCs were oriented and neurites extended along the length of the aligned fibers. SCs in co-culture with DRGs on PVDF-TrFE scaffolds promoted longer neurite extension as compared to scaffolds without SCs. In addition to promoting neurite extension, SCs also formed myelin around DRG neurites on PVDF-TrFE scaffolds. This study demonstrated PVDF-TrFE scaffolds containing aligned fibers supported SC-neurite extension and myelination. The combination of SCs and PVDF-TrFE scaffolds may be a promising tissue engineering strategy for spinal cord repair.
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影响因子：
14
作者：
FINE, EG;VALENTINI, RF;AEBISCHER, P
通讯作者：
AEBISCHER, P
DOI：
10.1073/pnas.80.12.3850
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CORNBROOKS, CJ;CAREY, DJ;BUNGE, RP
通讯作者：
BUNGE, RP
影响因子：
14
作者：
Chew, Sing Ylan;Mi, Ruifa;Leong, Kam W.
通讯作者：
Leong, Kam W.
影响因子：
7.8
作者：
CAREY, DJ;ELDRIDGE, CF;BUNGE, RP
通讯作者：
BUNGE, RP
影响因子：
2.5
作者：
Fortun J;Hill CE;Bunge MB
通讯作者：
Bunge MB