Examination of capillary zone electrophoresis, capillary isoelectric focusing and sodium dodecyl sulfate capillary electrophoresis for the analysis of recombinant tissue plasminogen activator.
Examination of capillary zone electrophoresis, capillary isoelectric focusing and sodium dodecyl sulfate capillary electrophoresis for the analysis of recombinant tissue plasminogen activator.
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毛细管区带电泳、毛细管等电聚焦和十二烷基硫酸钠毛细管电泳检查用于分析重组组织纤溶酶原激活剂。
DOI：
10.1016/0021-9673(96)00513-4
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Karger,BL
中科院分区：
文献类型：
--
作者：
Thorne,JM;Goetzinger,WK;Chen,AB;Moorhouse,KG;Karger,BL
用毛细管电泳法、毛细管电泳法和毛细管电泳法对重组组织型纤溶酶原激活剂(RtPA)这一复杂糖蛋白进行了微尺度分析。发现一系列θ-氨基酸缓冲液(pH≈5)适用于涂布毛细管上rtPA的毛细管电泳法分离。在含有0.01%(v/v)吐温80的ϵ-氨基己酸缓冲液中，rTPA可分解成一系列主峰和次峰。对于CIEF，采用压力动员的两步法。使用商用仪器，要么是带有50μ微米直径的聚合物溶液，要么是不含聚合物溶液的窄口径毛细管(25μ微米直径)。都被雇佣了。在等电点6.4-9.2的范围内，用Ampholine 3.5-10将RTPA至少拆分成8个物种。主峰的迁移时间精密度为0.2%(CZE)和2-3%(≤)(CIEF)。两种方法均可从毛细管中完全回收rtPA。对rtPA、rtPA I型、rtPA II型和所需形式的分析得到了预期的洗脱曲线。最后，展示了使用涂覆毛细管的十二烷基硫酸钠毛细管电泳法检测rtPA I/II纯度的潜力。
The microscale techniques of CZE, cIEF and SDS capillary electrophoresis have been evaluated for the analysis of a complex glycoprotein, recombinant tissue plasminogen activator (rtPA). A series of θ-amino acid buffers (pH≈5) was found suitable for the CZE separation of rtPA on coated capillaries. rtPA could be resolved into a series of major and minor peaks in an ϵ-aminocaproic acid buffer containing 0.01% (v/v) Tween 80. For cIEF, a two step method with pressure mobilization was utilized. Using a commercial instrument, either a polymer solution with a 50 μm I.D. capillary or narrow bore capillaries without a polymer solution (25 μm I.D.) were employed. rtPA was resolved into at least eight species within a pI range of 6.4–9.2 using Ampholine 3.5–10. Migration time precision for the major peaks ranged from 0.2% for CZE to ≤2–3% R.S.D. for cIEF. Total recovery of rtPA from the capillary was also demonstrated for both methods. Analysis of rtPA, rtPA Type I, rtPA Type II and the desialylated forms resulted in the expected elution profiles. Finally, the potential of SDS capillary electrophoresis using a coated capillary for an rtPA Type I/Type II purity assay was shown.
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影响因子：
2.9
作者：
N. Watts;R. Singh
通讯作者：
R. Singh
ε-氨基-正己酸对循环血液中纤溶酶激活产生的狗出血的抑制作用
DOI：
10.2302/kjm.8.247
发表时间：
1959
期刊：
The Keio Journal of Medicine
影响因子：
--
作者：
S. Okamoto;T. Nakajima;U. Okamoto;Hidefumi Watanabe;Yutaka Iguchi;T. Igawa;C. Chien;Takashi Hayashi
通讯作者：
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影响因子：
2.9
作者：
MOLTENI, S;FRISCHKNECHT, H;THORMANN, W
通讯作者：
THORMANN, W
影响因子：
4.1
作者：
MOORHOUSE, KG;EUSEBIO, CA;CHEN, AB
通讯作者：
CHEN, AB
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影响因子：
2.9
作者：
M. Taverna;A. Baillet;D. Biou;M. Schlüter;R. Werner;D. Ferrier
通讯作者：
D. Ferrier