miR-133b Promotes Esophageal Squamous Cell Carcinoma Metastasis.
miR-133b Promotes Esophageal Squamous Cell Carcinoma Metastasis.
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DOI：
10.1177/11795549231219502
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发表时间：
2023
影响因子：
2.2
通讯作者：
Lu, Xiao-Mei
中科院分区：
文献类型：
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作者：
Huang, Cong-Gai;Liu, Qing;Zheng, Shu-Tao;Liu, Tao;Tan, Yi-Yi;Peng, Tian-Yuan;Chen, Jiao;Lu, Xiao-Mei
在分子水平上评价食管鳞状细胞癌的生物学变化对提高食管鳞状细胞癌的生存率具有重要的临床意义。因此，我们计划分析和阐明microRNA-133 b（miR-133 b）、M2丙酮酸激酶（PKM 2）和信号转导和转录激活因子3（STAT 3）在食管鳞癌中的表达及其相关的临床病理意义。选取72例食管鳞癌患者作为实验组。正常邻近组织（NAT）作为对照组。本研究采用原位杂交法检测miR-133 b在食管鳞癌中的表达，采用免疫组化法检测PKM 2和STAT 3在食管鳞癌组织中的表达，并进行文献复习。有研究表明，NAT中miR-133 b的阳性表达显著高于ESCC（χ2 = 9.007，P = .003）。PKM 2和STAT 3在食管鳞癌中的阳性表达率明显高于NAT（χ2 = 56.523，P = .000; χ2 = 72.939，P = .000）。相关性分析显示，miR-133 b与PKM 2呈负相关（r =-0.515，P < .001），miR-133 b与STAT 3呈负相关（r =-0.314，P = .007），PKM 2与STAT 3呈正相关（r = 0.771，P < .001）。在ESCC中，我们的研究表明miR-133 b的下调和PKM 2和STAT 3的上调。我们预测miR-133 b可能通过下调PKM 2来抑制STAT 3通路。
Evaluation of biological changes at the molecular level has important clinical implications for improving the survival rate of esophageal squamous cell carcinoma (ESCC). Therefore, we plan to analyze and elucidate the expression of microRNA-133b (miR-133b), M2 pyruvate kinase (PKM2), and signal transducer and activator of transcription 3 (STAT3) in ESCC and their associated clinicopathological significance. The 72 patients with ESCC were selected as the experimental study group. Normal adjacent tissues (NAT) were matched as the control group. In this study, in situ hybridization was used to detect the expression of miR-133b in ESCC, and tissue expressions of PKM2 and STAT3 were detected by immunohistochemistry, and literature review was conducted. Studies had shown that the positive expression of miR-133b in NAT was significantly higher than that in ESCC (χ2 = 9.007, P = .003). PKM2 and STAT3 in ESCC had a significantly higher positive expression levels than those of NAT (χ2 = 56.523, P = .000; χ2 = 72.939, P = .000). From correlation analysis, there was a negative correlation between miR-133b and PKM2(r = −0.515, P < .001), a negative correlation between miR-133b and STAT3(r = −0.314, P = .007), and a positive correlation between PKM2 and STAT3(r = 0.771, P < .001). In ESCC, our study demonstrated that downregulation of miR-133b and upregulation of PKM2 and STAT3. We predict that miR-133b may inhibit the STAT3 pathway by downregulating PKM2.
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影响因子：
4
作者：
Ma, Rong;Liu, Qing;Lu, Xiaomei
通讯作者：
Lu, Xiaomei
影响因子：
7.3
作者：
Gu J;Xu H;Chen Y;Li N;Hou X
通讯作者：
Hou X
影响因子：
4.6
作者：
Taniguchi K;Ito Y;Sugito N;Kumazaki M;Shinohara H;Yamada N;Nakagawa Y;Sugiyama T;Futamura M;Otsuki Y;Yoshida K;Uchiyama K;Akao Y
通讯作者：
Akao Y
影响因子：
2.7
作者：
Cheng, Nan;Wang, Guang-Hui
通讯作者：
Wang, Guang-Hui
DOI：
10.1002/cac2.12457
发表时间：
2023-09
期刊：
Cancer communications (London, England)
影响因子：
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作者：
通讯作者：
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