High diversity of airborne fungi in the hospital environment as revealed by meta-sequencing-based microbiome analysis.
High diversity of airborne fungi in the hospital environment as revealed by meta-sequencing-based microbiome analysis.
复制标题
基于元测序的微生物组分析揭示了医院环境中空气传播真菌的高度多样性。
DOI：
10.1038/srep39606
复制
发表时间：
2017-01-03
影响因子：
4.6
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Tong X;Xu H;Zou L;Cai M;Xu X;Zhao Z;Xiao F;Li Y
在医院获得的侵袭性真菌感染已逐渐成为危及生命的感染的重要原因。特别是，医院中的空气传播真菌被认为是医院相关感染的关键病原体。为了鉴定致病性空气微生物，使用大容量空气采样器进行收集，并使用基于培养的方法和使用Illumina MiSeq和HiSeq 2000测序系统的DNA测序分析进行物种鉴定。在血琼脂中培养后几乎没有细菌生长。然而，使用微生物组测序，确定了真菌、真菌属物种、细菌和病毒的相对丰度。采用热图分析法对呼吸重症监护病房、重症监护病房、急诊室和门诊4个科室的真菌分布特征进行调查。真菌中曲霉属的患病率在种水平上最高，约为17%至61%，而烟曲霉属的患病率在四个部门中为34%至50%。从医院环境中分离的微生物的基因组草图，通过序列分析，表明空气中的真菌多样性的调查可能会提供可靠的结果，为医院感染控制和监测。
Invasive fungal infections acquired in the hospital have progressively emerged as an important cause of life-threatening infection. In particular, airborne fungi in hospitals are considered critical pathogens of hospital-associated infections. To identify the causative airborne microorganisms, high-volume air samplers were utilized for collection, and species identification was performed using a culture-based method and DNA sequencing analysis with the Illumina MiSeq and HiSeq 2000 sequencing systems. Few bacteria were grown after cultivation in blood agar. However, using microbiome sequencing, the relative abundance of fungi, Archaea species, bacteria and viruses was determined. The distribution characteristics of fungi were investigated using heat map analysis of four departments, including the Respiratory Intensive Care Unit, Intensive Care Unit, Emergency Room and Outpatient Department. The prevalence of Aspergillus among fungi was the highest at the species level, approximately 17% to 61%, and the prevalence of Aspergillus fumigatus among Aspergillus species was from 34% to 50% in the four departments. Draft genomes of microorganisms isolated from the hospital environment were obtained by sequence analysis, indicating that investigation into the diversity of airborne fungi may provide reliable results for hospital infection control and surveillance.
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影响因子：
5.1
作者：
Pyankov, Oleg V.;Agranovski, Igor E.;Khromykh, Alexander A.
通讯作者：
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影响因子：
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作者：
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DOI：
10.1016/s0924-8579(08)70009-8
发表时间：
2008-11-01
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作者：
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