Identification of recent cannabis use: whole-blood and plasma free and glucuronidated cannabinoid pharmacokinetics following controlled smoked cannabis administration.
Identification of recent cannabis use: whole-blood and plasma free and glucuronidated cannabinoid pharmacokinetics following controlled smoked cannabis administration.
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DOI：
10.1373/clinchem.2011.171777
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发表时间：
2011-10
影响因子：
9.3
通讯作者：
Huestis MA
中科院分区：
文献类型：
--
作者：
Schwope DM;Karschner EL;Gorelick DA;Huestis MA
Δ-9-四氢大麻酚(THC)是在事故调查和药物影响下驾驶的最常见的非法药物。四氢呋喃-葡萄糖醛酸苷被认为是最近使用大麻的一个标志，但在受控给药后没有血液数据来验证这一假设。此外，没有直接研究全血大麻素药代动力学的研究，尽管这种基质通常是唯一可用的样本。受试者(9名男性，1名女性)居住在一个封闭的研究单位，并随意吸食一支6.8%的大麻香烟。为避免稳定性问题，我们在采集后24小时内，用高效液相色谱/串联质谱法直接测定了全血和血浆中的THC、11-羟基-THC(11-OH-THC)、11-Nor-9-Carxy-THC(THCCOOH)、大麻二酚(CBD)、大麻酚(CBN)、THC-葡萄糖醛酸苷和THCCOOH-葡萄糖醛酸苷。开始吸烟0.25h后全血(血浆)最大浓度中位数(Cmax)分别为50(76)、6.4、41(67)、1.3(2.0)、2.4(3.6)、89(190)和0.7(1.4)μg/L；在观察Cmax时，CBD、CBN和THC-葡萄糖醛酸苷的全血(血浆)检出率分别为60%(80%)、80%(90%)和50%(80%)。在两种基质中均未检测到CBD和CBN(LOQ1.0μg/L)。吸食大麻后的人类全血大麻素数据将有助于对全血和血浆大麻素的解释，同时进一步确定最近的大麻摄入量。
Δ9-Tetrahydrocannabinol (THC) is the most frequently observed illicit drug in investigations of accidents and driving under the influence of drugs. THC-glucuronide has been suggested as a marker of recent cannabis use, but there are no blood data following controlled THC administration to test this hypothesis. Furthermore, there are no studies directly examining whole-blood cannabinoid pharmacokinetics, although this matrix is often the only available specimen. Participants (9 men, 1 woman) resided on a closed research unit and smoked one 6.8% THC cannabis cigarette ad libitum. We quantified THC, 11-hydroxy-THC (11-OH-THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), cannabinol (CBN), THC-glucuronide and THCCOOH-glucuronide directly in whole blood and plasma by liquid chromatography/ tandem mass spectrometry within 24 h of collection to obviate stability issues. Median whole blood (plasma) observed maximum concentrations (Cmax) were 50 (76), 6.4, 41 (67), 1.3 (2.0), 2.4 (3.6), 89 (190), and 0.7 (1.4) μg/L 0.25 h after starting smoking for THC, 11-OH-THC, THCCOOH, CBD, CBN, and THCCOOH-glucuronide, respectively, and 0.5 h for THC-glucuronide. At observed Cmax, whole-blood (plasma) detection rates were 60% (80%), 80% (90%), and 50% (80%) for CBD, CBN, and THC-glucuronide, respectively. CBD and CBN were not detectable after 1 h in either matrix (LOQ 1.0 μg/L). Human whole-blood cannabinoid data following cannabis smoking will assist whole blood and plasma cannabinoid interpretation, while furthering identification of recent cannabis intake.
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影响因子：
2.5
作者：
HUESTIS, MA;HENNINGFIELD, JE;CONE, EJ
通讯作者：
CONE, EJ
影响因子：
6.1
作者：
MATSUNAGA, T;IWAWAKI, Y;YOSHIMURA, H
通讯作者：
YOSHIMURA, H
影响因子：
9.3
作者：
Schwilke, Eugene W.;Schwope, David M.;Huestis, Marilyn A.
通讯作者：
Huestis, Marilyn A.
影响因子：
2.9
作者：
PEREZREYES, M;OWENS, SM;DIGUISEPPI, S
通讯作者：
DIGUISEPPI, S
影响因子：
4.3
作者：
Schwope, David M.;Scheidweiler, Karl B.;Huestis, Marilyn A.
通讯作者：
Huestis, Marilyn A.