Protein-specific Raman imaging of glycosylation on single cells with zone-controllable SERS effect.-猫眼课题宝

DOI：

10.1039/c5sc03560k

发表时间：

2016-01-01

期刊：

Chemical science

影响因子：

8.4

通讯作者：

中科院分区：

化学1区

文献类型：

作者：

Chen Y;Ding L;Song W;Yang M;Ju H

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519952/

区域可控的 SERS 效应集成了纳米基底尺寸的控制，以匹配用于拉曼成像的蛋白质特异性聚糖的表达区域。使用两种新设计的纳米探针，提出了区域可控的 SERS 效应，用于细胞表面蛋白质特异性糖基化的拉曼成像。使用拉曼信号分子和二苯并环辛炔胺对 10 nm Au 纳米颗粒进行功能化而制备的信号探针表现出可忽略不计的 SERS 效应，并且可以通过点击反应识别和连接叠氮化物标记的聚糖。底物探针是适配体修饰的 30 或 40 nm Au 纳米颗粒，可以特异性识别目标蛋白，在目标蛋白上创建有效的 SERS 区域。通过控制底物探针的大小以匹配蛋白质特异性聚糖的表达区域，可以产生有效的 SERS 信号。该方法已成功用于MCF-7细胞表面靶蛋白EpCAM上唾液酸的原位成像，并用于监测药物治疗过程中蛋白质特异性糖基化的表达变化。区域控制的概念也可用于测量细胞表面上糖蛋白之间的距离。该协议在揭示糖基化相关的生物过程方面显示出希望。

A zone-controllable SERS effect integrates the controlling of nano-substrate size to match the expression zone of protein-specific glycan for Raman imaging. A zone-controllable SERS effect is presented for Raman imaging of protein-specific glycosylation on a cell surface using two types of newly designed nanoprobes. The signal probe, prepared using a Raman signal molecule and dibenzocyclooctyne-amine to functionalize a 10 nm Au nanoparticle, exhibits a negligible SERS effect and can recognize and link the azide-tagged glycan via a click reaction. The substrate probe, an aptamer modified 30 or 40 nm Au nanoparticles, can specifically recognize the target protein to create an efficient SERS zone on the target protein. By controlling the size of the substrate probe to match the expression zone of the protein-specific glycan, an efficient SERS signal can be generated. This method has been successfully used for in situ imaging of sialic acids on the target protein EpCAM on an MCF-7 cell surface and for the monitoring of the expression variation of protein-specific glycosylation during drug treatment. The concept of zone control can also be used to measure the distance between glycoproteins on a cell surface. This protocol shows promise in uncovering glycosylation-related biological processes.