Regulation of glucagon-like peptide-1-(7-36) amide, peptide YY, and neurotensin secretion by neurotransmitters and gut hormones in the isolated vascularly perfused rat ileum.
Regulation of glucagon-like peptide-1-(7-36) amide, peptide YY, and neurotensin secretion by neurotransmitters and gut hormones in the isolated vascularly perfused rat ileum.
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离体血管灌注大鼠回肠中神经递质和肠道激素对胰高血糖素样肽-1-(7-36)酰胺、肽 YY 和神经降压素分泌的调节。
DOI：
10.1210/endo.136.11.7588257
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发表时间：
1995
期刊：
影响因子：
4.8
通讯作者：
J. Cuber
中科院分区：
文献类型：
--
作者：
V. Dumoulin;T. Dakka;P. Plaisancié;J. Chayvialle;J. Cuber
神经降压素(NT)、多肽YY(PYY)和几种来源于胰高血糖素原的多肽在口服后迅速从肠道远端的内分泌细胞中释放出来，从而表明这些多肽的释放部分受到神经和/或激素的控制。我们之前在大鼠结肠血管灌流模型上进行的研究表明，结肠L细胞对肠道的几种递质和激素肽GIP具有高度的反应性。为探讨小肠近端或肠神经系统递质产生的激素是否也可调节回肠L细胞的分泌活动，在血管灌流的大鼠离体回肠标本上，动脉注射各种肠道调节肽和神经递质30min。比较回肠N细胞的分泌活性。用特异性放射免疫分析法检测门静脉流出液中NT、PYY和胰升糖素样肽-1(GLP-1)的释放。M胆碱能激动剂10(-4)M可引起PYY、GLP-1和NT的双相释放，包括一个早期峰值和一个持续的反应。同样，蛙皮素(10(-7)M)可诱导PYY和GLP-1的双相释放。相反，NT的反应基本上是单相的，以早期分泌高峰为特征。河豚毒素不能改变蛙皮素诱导的PYY、GLP-1和NT的释放。β-肾上腺素能激动剂异丙肾上腺素在10(-6)M时引起门脉PYY和GLP-1浓度的一过性升高，而对NT释放的影响明显是双相的。降钙素基因相关肽(5×10~(-8)M)可引起门静脉流出液中PYY、GLP-1和NT免疫反应活性显著升高(峰值分别为基础值的600%、500%和550%，给药后4min)。动脉内注射GIP的浓度范围(0.5-3 nM)仅在阈值浓度为3 nM时才能引起门静脉血中这三种多肽浓度的显著升高。促胰液素(50 Pm)或缩胆囊素(50 Pm)不影响回肠激素的释放。综上所述，回肠L和N细胞对肠道的各种递质做出反应。多肽的释放模式取决于所研究的细胞类型。在本研究的条件下，两个共合成的多肽PYY和GLP-1似乎是共分泌的。
Neurotensin (NT), peptide YY (PYY), and several peptides derived from proglucagon are promptly released from endocrine cells of the distal part of the gut after oral ingestion of a meal, thus suggesting that release of these peptides is partly under neural and/or hormonal control. Our previous studies conducted with a model of isolated vascularly perfused rat colon showed that colonic L cells are highly responsive to several transmitters of the gut and to the hormonal peptide GIP. To test the possibility that hormones produced by the proximal small intestine or transmitters of the enteric nervous system may also modulate the secretory activity of the ileal L cells, various intestinal regulatory peptides and neurotransmitters were administered intraarterially for 30 min in the isolated vascularly perfused rat ileum preparation. The secretory activity of the ileal N cells was comparatively assessed. The release of NT, PYY, and glucagon-like peptide-1 (GLP-1) in the portal effluent was measured with specific RIAs. The muscarinic cholinergic agonist bethanechol at a concentration of 10(-4) M provoked a biphasic release of PYY, GLP-1, and NT, consisting of an early peak followed by a sustained response. Similarly, bombesin (10(-7) M) induced a marked biphasic release of PYY and GLP-1. In contrast, the NT response was essentially monophasic, characterized by an early peak secretion. Tetrodotoxin did not modify the bombesin-induced release of PYY, GLP-1, and NT. The beta-adrenergic agonist isoproterenol at a concentration of 10(-6) M induced a transient rise in portal PYY and GLP-1 concentrations, whereas the effect on NT release was clearly biphasic. Calcitonin gene-related peptide (5 x 10(-8) M) induced a dramatic rise in PYY, GLP-1, and NT immunoreactivities in the portal effluent (peaks at 600%, 500%, and 550% of the basal values, respectively, 4 mi n after the start of infusion). Intraarterial infusion of GIP over the concentration range (0.5-3 nM) evoked a significant increase in portal concentration of the three peptides only at the threshold concentration of 3 nM. Secretin (50 pM) or cholecystokinin (50 pM) did not affect the release of ileal hormones. In conclusion, ileal L and N cells respond to a variety of transmitters of the gut. The pattern of peptide release depends on the cell type studied. The two cosynthesized peptides, PYY and GLP-1, appear to be cosecreted in the conditions of the present study.
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通过选择性灌注狗的空肠释放神经降压素。
影响因子：
29.4
作者：
Fujimura,M;Khalil,T;Sakamoto,T;GreeleyJr,GH;Salter,MG;TownsendJr,CM;Thompson,JC
通讯作者：
Thompson,JC
回肠在脂肪释放的神经降压素中的重要性。
影响因子：
3.8
作者：
Walker,JP;Fujimura,M;Sakamoto,T;GreeleyJr,GH;TownsendJr,CM;Thompson,JC
通讯作者：
Thompson,JC
犬肠道原代细胞培养物神经降压素释放的调节。
DOI：
10.1152/ajpgi.1986.250.3.g385
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Barber,DL;Buchan,AM;Walsh,JH;Soll,AH
通讯作者：
Soll,AH
肽 YY 抑制膳食刺激的胰腺和胃分泌。
DOI：
10.1152/ajpgi.1985.248.1.g118
发表时间：
1985
期刊：
The American journal of physiology
影响因子：
--
作者：
Pappas,TN;Debas,HT;Goto,Y;Taylor,IL
通讯作者：
Taylor,IL
犬肠内分泌细胞的生长抑素和毒蕈碱抑制：细胞机制。
DOI：
10.1152/ajpgi.1987.253.5.g684
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Barber,DL;Gregor,M;Soll,AH
通讯作者：
Soll,AH