The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences.
The two mRNAs encoding rat intestinal alkaline phosphatase represent two distinct nucleotide sequences.
复制标题
编码大鼠肠碱性磷酸酶的两个 mRNA 代表两个不同的核苷酸序列。
作者:
Michael Engle;D. H. Alpers
Rat duodenal mucosa contains two mRNAs (2.7 and 3.0 kb) encoding intestinal alkaline phosphatase (IAP), but the mechanism for their production has not been clear. By means of the polymerase chain reaction (PCR), we isolated a fragment that identifies a second rat IAP (rIAP-II), differing from the rIAP-I sequence in the coding and 3' untranslated regions and encoding a COOH-terminal sequence predicted to be hydrophilic. By means of probes unique to each sequence, rIAP-I identified the 2.7-kb mRNA, and rIAP-II the 3.0-kb mRNA. The different structures and differential regulation of the mRNAs after fat feeding demonstrate the presence of two rat IAP transcripts.
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DOI:
10.1152/ajpgi.1990.259.4.g618
发表时间:
1990
期刊:
The American journal of physiology
影响因子:
--
作者:
Eliakim,R;Becich,MJ;Green,K;Alpers,DH
通讯作者:
Alpers,DH
DOI:
10.1016/s0021-9258(18)68159-1
发表时间:
1988-10
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
P. Orchansky;J. Escobedo;L. Williams
通讯作者:
P. Orchansky;J. Escobedo;L. Williams
DOI:
10.1042/bj2440001
发表时间:
1987-05
期刊:
The Biochemical journal
影响因子:
--
作者:
Martin G. Low
通讯作者:
Martin G. Low
DOI:
10.1042/bj2340563
发表时间:
1986
期刊:
The Biochemical journal
影响因子:
--
作者:
Sussman,NL;Seetharam,S;Blaufuss,MC;Alpers,DH
通讯作者:
Alpers,DH