A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex.
A recognition-before-labeling strategy for sensitive detection of lung cancer cells with a quantum dot-aptamer complex.
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一种利用量子点-适体复合物灵敏检测肺癌细胞的先识别后标记策略。
DOI：
10.1039/c5an01145k
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发表时间：
2015-08
期刊：
影响因子：
4.2
通讯作者：
Wang, Kemin
中科院分区：
文献类型：
--
作者：
Li, Jing;Ji, Haining;Yang, Xiaohai;Wang, Kemin
以荧光量子点为信号单元，DNA适配体为识别元件，建立了一种高特异性的荧光标记前先标记策略，用于非小细胞肺癌A549细胞的灵敏检测。用于细胞成像和生物分析的QD-适体系统主要依赖于先标记后纯化的策略，其中适体首先用QD标记，然后将QD-适体缀合物作为整体用于特异性识别。在我们的策略中，首先使用适体识别靶细胞，然后依次加入荧光量子点以结合适体并照亮靶细胞。所提出的先修饰后标记策略不需要复杂的QD功能化过程，并且避免了空间位阻对适体构型的可能影响。同时，量子点具有很强的荧光和良好的光稳定性，也使该方法具有较高的信号背景比（S/B）。该方法简便、灵敏，为癌细胞的体外诊断提供了一种新的方法。
A highly specific recognition-before-labeling strategy has been developed for sensitive detection of non-small cell lung cancer A549 cells, by using fluorescent QDs as signal units and DNA aptamers as recognition elements. A QD-aptamer system used for cell imaging and bioanalysis mostly relies on the recognition-after-labeling strategy in which aptamers were firstly labeled with QDs and then the QD-aptamer conjugates as a whole were utilized for specific recognition. Here in our strategy, aptamers were used firstly to recognize target cells, and then fluorescent QDs were sequentially added to bind the aptamers and light the target cells. The proposed recognition-before-labeling strategy didn't require the complex process of QD functionalization, and avoided the possible impact on the aptamer configuration from steric hindrance. Meanwhile, QDs, with strong fluorescence and good photostability, also give this method a high signal-to-background ratio (S/B). The recognition-before-labeling strategy is simple and sensitive, suggesting a new method for in vitro diagnostic assays of cancer cells.
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影响因子：
3.4
作者：
C. Sánchez-Domínguez;Hugo Leonid Gallardo Blanco;A. A. Rodríguez-Rodríguez-A.-A.-Rodríguez-Rodríguez-1421319530;Andrea V. Vela-Gonzalez;M. Sánchez-Domínguez
通讯作者：
C. Sánchez-Domínguez;Hugo Leonid Gallardo Blanco;A. A. Rodríguez-Rodríguez-A.-A.-Rodríguez-Rodríguez-1421319530;Andrea V. Vela-Gonzalez;M. Sánchez-Domínguez
影响因子：
7.4
作者：
Jianbo Liu;Yan Liu;Xiaohai Yang;Kemin Wang;Qing Wang;Hui Shi;Li Li-Li
通讯作者：
Jianbo Liu;Yan Liu;Xiaohai Yang;Kemin Wang;Qing Wang;Hui Shi;Li Li-Li
影响因子：
10.8
作者：
Pathak, Smita;Davidson, Marie C.;Silva, Gabriel A.
通讯作者：
Silva, Gabriel A.
影响因子：
6.4
作者：
Sefah, Kwame;Bae, Kyung-Mi;Phillips, Joseph A.;Siemann, Dietmar W.;Su, Zhen;McClellan, Steve;Vieweg, Johannes;Tan, Weihong
通讯作者：
Tan, Weihong
影响因子：
3.9
作者：
Algar, W. Russ;Krull, Ulrich J.
通讯作者：
Krull, Ulrich J.