Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes.
Use of a multiplexed CMOS microarray to optimize and compare oligonucleotide binding to DNA probes synthesized or immobilized on individual electrodes.
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DOI：
10.3390/s100807371
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Danley D
中科院分区：
文献类型：
--
作者：
Maurer K;Yazvenko N;Wilmoth J;Cooper J;Lyon W;Danley D
CombiMatrix微阵列具有12，544个电极，支持用户自定义DNA探针的原位电化学合成。作为替代方案，我们将商业合成的DNA探针固定在涂有电聚合聚吡咯（Ppy）的单个电极上。使用生物素化的靶寡核苷酸和Cy 5-链霉亲和素和荧光检测或辣根过氧化物酶-链霉亲和素和酶增强电化学检测来测量杂交。通过改变Ppy的沉积、DNA探针上的末端基团以及影响荧光猝灭和电导率的其他因素来优化检测效率。将优化的结果与使用具有原位合成的相同DNA序列的微阵列获得的结果进行比较。固定化探针产生更高的荧光信号，可能是通过在靶寡核苷酸上的Cy 5与Ppy和铂电极的淬灭效应之间提供更大的间隔。
The CombiMatrix microarray with 12,544 electrodes supports in situ electrochemical synthesis of user-defined DNA probes. As an alternative, we immobilized commercially synthesized DNA probes on individual electrodes coated with electropolymerized polypyrrole (Ppy). Hybridization was measured using a biotinylated target oligonucleotide and either Cy5-streptavidin and fluorescence detection or horseradish peroxidase-streptavidin and enzyme-enhanced electrochemical detection. Detection efficiencies were optimized by varying the deposition of the Ppy, the terminal groups on the DNA probes, and other factors that impacted fluorescence quenching and electrical conductivity. Optimized results were compared against those obtained using a microarray with the same DNA sequences synthesized in situ. Immobilized probes produced higher fluorescence signals, possibly by providing a greater stand off between the Cy5 on the target oligonucleotide and the quenching effects of the Ppy and the platinum electrode.
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DOI：
10.3390/s8010118
发表时间：
2008-01-09
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Rahman MA;Kumar P;Park DS;Shim YB
通讯作者：
Shim YB
影响因子：
14.9
作者：
LIVACHE, T;ROGET, A;TEOULE, R
通讯作者：
TEOULE, R
影响因子：
12.6
作者：
Ghindilis, Andrey L.;Smith, Maria W.;McShea, Andy
通讯作者：
McShea, Andy
DOI：
10.1081/ma-100108381
发表时间：
2001-01-01
期刊：
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
影响因子：
--
作者：
Minehan, DS;Marx, KA;Tripathy, SK
通讯作者：
Tripathy, SK
影响因子：
2.9
作者：
Livache, T;Fouque, B;Mathis, G
通讯作者：
Mathis, G