Structural and mechanical effects of in vivo fatigue damage induction on murine tendon.
Structural and mechanical effects of in vivo fatigue damage induction on murine tendon.
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DOI：
10.1002/jor.22012
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发表时间：
2012-06
影响因子：
2.8
通讯作者：
Flatow, Evan L.
中科院分区：
文献类型：
--
作者：
Sereysky, Jedd B.;Andarawis-Puri, Nelly;Jepsen, Karl J.;Flatow, Evan L.
本研究的目的是开发和验证肌腱疲劳的体内小鼠模型，并使用该模型来研究和量化小鼠肌腱疲劳损伤的物理表现。将C57 BL/6 J小鼠的髌腱以2 Hz疲劳加载至三个终点（4 N峰值力/循环持续1小时、6 N持续1小时和4 N持续2小时），在此期间测量每个循环的滞后、切线刚度和峰值应变。然后使用原位组织学定量损伤累积，并将每个肌腱单调加载至失效。所有三组的组织学损伤均显著增加（≥2倍），6 N，1 h和4 N，2 h组的单调刚度显著降低（~25%），表明损伤最初表现为肌腱胶原结构的变化，随后表现为功能的变化。对于本研究中使用的疲劳加载协议，没有一个评估的实时参数与损伤面积分数测量的结构损伤或单调刚度相关，这表明它们不适合作为损伤累积的代理。在未来的研究中，该模型将用于比较小鼠肌腱对不同遗传品系疲劳损伤的生物学反应。
The purpose of this study was to develop and validate an in vivo mouse model of tendon fatigue and use this model to investigate and quantify the physical manifestations of fatigue damage in mouse tendon. Patellar tendons of C57BL/6J mice were fatigue loaded at 2 Hz to three endpoints (4 N peak force per cycle for 1 h, 6 N for 1 h, and 4 N for 2 h), during which hysteresis, tangent stiffness, and peak strain of each cycle were measured. Damage accumulation was then quantified using in situ histology, and each tendon was loaded monotonically to failure. Histological damage increased significantly in all three groups (≥2-fold), and monotonic stiffness decreased significantly in the 6 N, 1 h and 4 N, 2-h groups (~25%), suggesting that damage initially manifests as changes to the collagen structure of the tendon and subsequently as changes to the function. For the fatigue loading protocols used in this study, none of the evaluated real-time parameters from fatigue loading correlated with damage area fraction measured structural damage or monotonic stiffness, suggesting that they are not suited to serve as proxies for damage accumulation. In future studies, this model will be used to compare the biological response of mouse tendon to fatigue damage across genetic strains.
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DOI：
10.1002/jor.20878
发表时间：
2009-10
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Szczodry M;Zhang J;Lim C;Davitt HL;Yeager T;Fu FH;Wang JH
通讯作者：
Wang JH
DOI：
10.1002/jor.20722
发表时间：
2009-02
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Fung DT;Wang VM;Laudier DM;Shine JH;Basta-Pljakic J;Jepsen KJ;Schaffler MB;Flatow EL
通讯作者：
Flatow EL
影响因子：
--
作者：
Scott, Alexander;Cook, Jill L.;Khan, Karim M.
通讯作者：
Khan, Karim M.
影响因子：
2.4
作者：
Fung, David T.;Wang, Vincent M.;Andarawis-Puri, Nelly;Basta-Pljakic, Jelena;Li, Yonghui;Laudier, Damien M.;Sun, Hui B.;Jepsen, Karl J.;Schaffler, Mitchell B.;Flatow, Evan L.
通讯作者：
Flatow, Evan L.
影响因子：
2.4
作者：
Pattin, CA;Caler, WE;Carter, DR
通讯作者：
Carter, DR