Early response to tendon fatigue damage accumulation in a novel in vivo model.
Early response to tendon fatigue damage accumulation in a novel in vivo model.
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DOI：
10.1016/j.jbiomech.2009.08.039
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发表时间：
2010-01-19
影响因子：
2.4
通讯作者：
Flatow, Evan L.
中科院分区：
文献类型：
--
作者：
Fung, David T.;Wang, Vincent M.;Andarawis-Puri, Nelly;Basta-Pljakic, Jelena;Li, Yonghui;Laudier, Damien M.;Sun, Hui B.;Jepsen, Karl J.;Schaffler, Mitchell B.;Flatow, Evan L.
关键词：
本研究描述了一种新的大鼠髌腱机械疲劳模型的开发和应用，用于研究肌腱亚失效损伤的早期体内反应。使用定制设计的加载装置，对成年雌性Sprague-Dawley大鼠的髌腱施加1-35 N的疲劳载荷。髌骨肌腱受到低、中或高水平疲劳损伤，通过夹持应变测量确定。分子反应与撕裂修复损伤进行了比较。组织学分析表明，肌腱疲劳的进展涉及在低损伤时形成局部扭结纤维变形，在中等损伤时随着纤维分层的存在而密度增加，在高损伤水平时纤维成角和不连续。在疲劳后第1天和第3天进行的RT-PCR分析显示，在低和中度损伤水平下，I型、III型和V型胶原mRNA表达发生了可变变化，与肌腱病理学的临床结果一致，并且与在高损伤水平下观察到的变化相比是适度的，在高损伤水平下，所有评估的胶原表达均显著增加。相比之下，只有I型胶原蛋白的表达升高，在同一时间点后撕裂。研究结果表明，累积疲劳肌腱调用不同的分子反应比撕裂伤。此外，结构修复可能直到达到终期疲劳寿命才开始，其中修复响应可能无法将损坏的钢筋束恢复到其疲劳前的结构。
This study describes the development and application of a novel rat patellar tendon model of mechanical fatigue for investigating the early in vivo response to tendon subfailure injury. Patellar tendons of adult female Sprague-Dawley rats were fatigue loaded between 1–35 N using a custom-designed loading apparatus. Patellar tendons were subjected to Low-, Moderate- or High-level fatigue damage, defined by grip-to-grip strain measurement. Molecular response was compared with that of a laceration-repair injury. Histological analyses showed that progression of tendon fatigue involves formation of localized kinked fiber deformations at Low damage, which increased in density with presence of fiber delaminations at Moderate damage, and fiber angulation and discontinuities at High damage levels. RT-PCR analysis performed at 1- and 3-day post-fatigue showed variable changes in type I, III and V collagen mRNA expression at Low and Moderate damage levels, consistent with clinical findings of tendon pathology and were modest compared with those observed at High damage levels, in which expression of all collagens evaluated were increased markedly. In contrast, only type I collagen expression was elevated at the same time points post-laceration. Findings suggest that cumulative fatigue in tendon invokes a different molecular response than laceration. Further, structural repair may not be initiated until reaching end-stage fatigue life, where the repair response may unable to restore the damaged tendon to its pre-fatigue architecture.
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DOI：
10.1590/s1807-59322008000100003
发表时间：
2008-02
期刊：
Clinics (Sao Paulo, Brazil)
影响因子：
--
作者：
Satomi E;Teodoro WR;Parra ER;Fernandes TD;Velosa AP;Capelozzi VL;Yoshinari NH
通讯作者：
Yoshinari NH
影响因子：
2.8
作者：
Laudier, Damien;Schaffler, Mitchell B.;Wang, Vincent M.
通讯作者：
Wang, Vincent M.
影响因子：
2.3
作者：
Hiranuma, K;Suzuki, K;Hirano, H
通讯作者：
Hirano, H
影响因子：
3
作者：
Sonnabend, DH;Yu, Y;Walsh, WR
通讯作者：
Walsh, WR
影响因子：
2.4
作者：
Ralphs, JR;Benjamin, M;Gao, J
通讯作者：
Gao, J