Treadmill running exercise results in the presence of numerous myofibroblasts in mouse patellar tendons.
Treadmill running exercise results in the presence of numerous myofibroblasts in mouse patellar tendons.
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DOI：
10.1002/jor.20878
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发表时间：
2009-10
期刊：
影响因子：
--
通讯作者：
Wang JH
中科院分区：
文献类型：
--
作者：
Szczodry M;Zhang J;Lim C;Davitt HL;Yeager T;Fu FH;Wang JH
已知机械载荷会改变肌腱结构，但其细胞机制尚不清楚。本研究旨在确定机械载荷对体内肌腱细胞的影响。采用C57BL/6J雌性小鼠进行跑步机跑步实验。跑步机跑步方案包括跑步机训练一周，然后以13米/分钟的速度持续适度跑步50分钟/天，每周5天，持续3周。对跑步后的小鼠肌腱切片进行免疫组化染色，发现肌腱切片中有大量细胞表达α-SMA，而在对照组小鼠肌腱切片中，只有少数细胞表现出微弱的α-SMA信号。此外，与对照组小鼠的mptc相比，来自跑步小鼠的小鼠髌骨肌腱细胞（mptc）在培养中普遍更大，增殖更快，表达更高水平的α-SMA，形成更丰富的应力纤维。此外，跑步机小鼠的mptc产生的牵引力（169±66.1 Pa）大于对照组小鼠（102±34.2 Pa）。最后，跑步机小鼠的细胞产生的总胶原蛋白水平（516.4±92.7µg/10,000细胞）高于对照组（303.9±34.8µg/10,000细胞）。因此，通过跑步机跑步的机械负荷增加了小鼠髌骨肌腱中肌成纤维细胞的存在。由于肌成纤维细胞是被激活的成纤维细胞，它们在跑步机跑步后出现在肌腱中，表明它们在剧烈的机械负荷下积极修复和重塑肌腱组织，导致已知的肌腱结构变化。
Mechanical loading is known to alter tendon structure, but its cellular mechanisms are unclear. This study aimed to determine the effect of mechanical loading on tendon cells in vivo. C57BL/6J female mice were used in a treadmill running study. The treadmill running protocol consisted of treadmill training for one week, followed by sustained moderate running at 13 m/min for 50 min/day, 5 days/week, for 3 weeks. Immunohistochemical staining of tendon sections of mice after treadmill running revealed that numerous cells in the tendon section expressed α-SMA, whereas in the tendon sections of control mice, only a few cells exhibited weak α-SMA signals. Furthermore, mouse patellar tendon cells (MPTCs) derived from treadmill running mice were generally larger in culture, proliferated faster, expressed a higher level of α-SMA, and formed more abundant stress fibers compared to MPTCs from control mice. In addition, MPTCs from treadmill running mice generated larger traction forces (169 ± 66.1 Pa) than those from control mice (102 ± 34.2 Pa). Finally, cells from treadmill running mice produced higher levels of total collagen (516.4 ± 92.7 µg/10,000 cells) than their counterparts (303.9± 34.8 µg/10,000 cells). Thus, mechanical loading via treadmill running increased the presence of myofibroblasts in mouse patellar tendons. As myofibroblasts are activated fibroblasts, their presence in the tendon following treadmill running indicates that they actively repair and remodel tendon tissue under strenuous mechanical loading, leading to known changes in tendon structure.
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影响因子：
8.3
作者：
Petrov, VV;Fagard, RH;Lijnen, PJ
通讯作者：
Lijnen, PJ
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.
影响因子：
--
作者：
Chen, Jianxin;Li, Hongxia;Wang, James H. -C.
通讯作者：
Wang, James H. -C.
影响因子：
18.4
作者：
Gibbon, WW;Cooper, JR;Radcliffe, GS
通讯作者：
Radcliffe, GS
DOI：
10.1164/rccm.200509-1381oc
发表时间：
2006-06-01
影响因子：
24.7
作者：
Broecker, Verena;Laenger, Florian;Lehmann, Ulrich
通讯作者：
Lehmann, Ulrich