Indirect micromanipulation of single molecules in water‐in‐oil emulsion
Indirect micromanipulation of single molecules in water‐in‐oil emulsion
复制标题
油包水乳液中单分子的间接显微操作
DOI：
10.1002/1522-2683(200101)22:2
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发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
A. Mizuno
中科院分区：
文献类型：
--
作者：
S. Katsura;A. Yamaguchi;Hiroyoshi Inami;S. Matsuura;K. Hirano;A. Mizuno
基于真实的实时观察和显微操作，单DNA分子的分析方法已经开发了一段时间。然而，精确的操作仍然很困难，因为单分子对于常规技术来说太小了。我们开发了一种化学反应系统，使用油中的水滴作为材料的容器。水滴可以通过光学力来操纵。水滴的操作允许两个选定的水滴融合。该过程对应于不同样品的混合。我们将该系统命名为“w/o（油包水乳液）微反应器系统”，并且每个液滴可以被认为是“微反应器”。在这个系统中，可以很容易地操作单个分子，因为分子可以包含在μm大小的微反应器中。微反应器使用极少量的样品，因此，反应是快速的，因为微反应器中的扩散时间非常短。基于光学力的微反应器操纵技术已被应用于诱导载有DNA的微反应器与YOYO（一种与DNA结合的荧光染料）之间的融合。这种融合诱导YOYO的快速结合。
Based on real‐time observation and micromanipulation, analytical methods for single DNA molecules have been under development for some time. Precise manipulation, however, is still difficult because single molecules are too small for conventional techniques. We have developed a chemical reaction system that uses water droplets in oil as containers of materials. The water droplets can be manipulated by optical force. The manipulation of the water droplets permits the fusion of two selected droplets. This process corresponds to mixing of different samples. We designate this system as “w/o (water‐in‐oil emulsion) microreactor system”, and each droplet can be thought of as a “microreactor”. In this system, single molecules can be manipulated readily, as a molecule can be contained in a μm‐sized microreactor. The microreactor utilizes extremely small quantities of samples, therefore, reactions are rapid, as diffusion times in the microreactor are very short. The manipulation technique of the microreactors based on optical force has been applied to induce fusion between microreactors loaded with DNA and YOYO, a fluorescent dye that binds to DNA. This fusion induced a rapid binding of YOYO.
影响因子：
56.9
作者：
Chiu, DT;Wilson, CF;Zare, RN
通讯作者：
Zare, RN
影响因子：
7.4
作者：
Chiu, DT;Hsiao, A;Zare, RN
通讯作者：
Zare, RN