Phospholipid spin probes measure the effects of ethanol on the molecular order of liver microsomes.
Phospholipid spin probes measure the effects of ethanol on the molecular order of liver microsomes.
复制标题
磷脂自旋探针测量乙醇对肝微粒体分子顺序的影响。
DOI：
10.1021/bi00346a012
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Rubin,E
中科院分区：
文献类型：
--
作者：
Taraschi,TF;Wu,A;Rubin,E
Ethanol, in vitro, is known to perturb the molecular order of the phospholipids in biological membranes, while chronic ethanol exposure, in vivo, leads to resistance to disordering. Such changes have usually been measured byelectron spin resonance, utilizing fatty acid spin probes. The use of such probes is controversial, since their orientation in the membrane may not accurately represent that of individual phospholipids. We, therefore, compared ethanol-induced structural perturbations in the membranes of rat hepatic microsomes measured with the spin probe 12-doxylstearic acid (SA 12) with those assayed with various phospholipid spin probes. With SA 12, the addition of increasing amounts of ethanol (50-250 mM) in vitro caused a progressive decrease in the membrane molecular order, as measured by electron spin resonance(ESR). By contrast, microsomes obtained from rats chronically fed ethanol were resistant to the disordering effect of ethanol. Microsomes labeled with the phospholipid spin probes l-palmitoyl-2-(12-doxylstearoyl) phosphatidylcholine,-phosphatidylethanolamine, or-phosphatidic acid also exhibited increased disordering with the addition of increasing amounts of ethanol. However, the effect noted with phospholipid spin probes was less than that observed with the fatty acid probe. Microsomes obtained from the livers of chronically intoxicated animalslabeled with the phospholipid probes were also resistant to the disordering effects of ethanol in vitro. These results suggest that (1) fatty acid spin probes are qualitatively valid for measuring membrane perturbations in biological membranes,(2) ethanol affects all microsomal phospholipids, regardless of chemical dissimilarities (eg, head-group structure), in a qualitatively similar fashion, and (3) the fluidization of fatty acyl chains in microsomal membranes is comparable in different membrane phospholipids.Increasing evidence from this (Rottenberg et al., 1981; Waring et al., 1981, 1982) and other laboratories (Chin & Goldstein, 1977a, 1981; Harris & Schroeder, 1981) suggests that thedeleterious effects of chronic ethanol intoxication may be related to changes that occur at the level of cell membranes. Incubation with ethanol in vitro has been shownby electron spin resonance(ESR) 1 to cause molecular disordering of brain synaptic membranes (Harris & Schroeder, 1981; Chin & Goldstein, 1977b), liver mitochondria (Waring et al., 1981, 1982) and microsomes (Ponnappa et al., 1982), and red blood cell membranes (Chin & Goldstein, 1977b). Similar changes have been reported for synaptic plasma membranes instudies utilizing fluorescence polarization (Harris & Schroeder, 1981). In contrast to the fluidizing effect of ethanol on biological membranes in vitro, chronic ethanol intoxication causes re-sistance to the disordering effects of ethanol and, in some cases, has been reported to increase membrane molecular order, as first reported by Chin & Goldstein (1977a) for synaptic membranes. The resistance to disordering has subsequently also been observed in liver mitochondria (Rottenberg et al., 1981; Waring et al., 1981, 1982) and microsomes (Ponnappa et al., 1982), erythrocyte ghosts (Chin & Goldstein, 1977a; Kelly-Murphy et al., 1984), and pancreatic plasma membranes (Ponnappa et al., 1982). Recent studies from this laboratory have demonstratedthat chronic ethanol treatment decreases the partitioning of ethanoland other lipophilic compounds into a variety of biological membranes (Waring et al., 1981; Kelly-Murphy et al., 1984).
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乙醇的膜紊乱作用：随膜胆固醇含量和自旋标记探针深度的变化。
影响因子：
3.6
作者：
Chin,JH;Goldstein,DB
通讯作者：
Goldstein,DB
长期服用乙醇会诱导豚鼠突触质膜中磷脂酰丝氨酸的增加。
DOI：
10.1016/0006-291x(83)90460-6
发表时间：
1983
影响因子：
3.1
作者：
Sun,GY;Sun,AY
通讯作者：
Sun,AY
影响因子：
3.6
作者：
R. Harris;F. Schroeder
通讯作者：
F. Schroeder
慢性酗酒者的耐受性和交叉耐受性：乙醇和其他药物的膜结合减少。
DOI：
10.1126/science.6264608
发表时间：
1981
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rottenberg,H;Waring,A;Rubin,E
通讯作者：
Rubin,E
慢性乙醇消耗对肝线粒体膜温度依赖性物理特性的影响。
影响因子：
3.9
作者：
Waring,AJ;Rottenberg,H;Ohnishi,T;Rubin,E
通讯作者：
Rubin,E