Early developing pig embryos mediate their own environment in the maternal tract.
Early developing pig embryos mediate their own environment in the maternal tract.
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DOI：
10.1371/journal.pone.0033625
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fazeli A
中科院分区：
文献类型：
--
作者：
Almiñana C;Heath PR;Wilkinson S;Sanchez-Osorio J;Cuello C;Parrilla I;Gil MA;Vazquez JL;Vazquez JM;Roca J;Martinez EA;Fazeli A
母体管道在早期胚胎发育的成功中起着关键作用，为建立和维持妊娠提供了最佳环境。这种环境的准备需要胚胎和母亲之间的亲密对话。然而，在这个独特的通信系统中，许多有趣的方面仍然是未知的。为了促进我们对胚泡被子宫内膜接受的过程的理解，并更好地解决不孕症和妊娠失败的临床挑战，必须破译这种复杂的分子对话。目前的工作的目的是确定当地的反应，对胚胎在怀孕的最早阶段的母亲道。我们使用了一种新的体内实验模型，消除了遗传变异性和个体差异，然后使用Affyphin微阵列来识别参与这种胚胎-母体对话的信号。采用腹腔镜授精技术，对母猪一侧输卵管注入精子，对侧输卵管注入稀释液。该模型使我们能够从输卵管和子宫角尖端获得含有来自同一母猪的胚胎或卵母细胞的样品。微阵列分析表明，大多数差异表达的转录物在子宫角中的表达下调，以响应胚泡相比，卵母细胞。许多在子宫角中对胚胎反应的转录物改变与免疫系统有关。我们使用计算机数学模型来证明胚胎作为免疫系统调节剂的作用。该模型显示，胚胎的存在引起的相对适度的变化可以调节母体的免疫反应。这些发现表明，胚胎的存在可能会调节母体的免疫系统，使难治性子宫能够耐受胚胎并支持其发育。
The maternal tract plays a critical role in the success of early embryonic development providing an optimal environment for establishment and maintenance of pregnancy. Preparation of this environment requires an intimate dialogue between the embryo and her mother. However, many intriguing aspects remain unknown in this unique communication system. To advance our understanding of the process by which a blastocyst is accepted by the endometrium and better address the clinical challenges of infertility and pregnancy failure, it is imperative to decipher this complex molecular dialogue. The objective of the present work is to define the local response of the maternal tract towards the embryo during the earliest stages of pregnancy. We used a novel in vivo experimental model that eliminated genetic variability and individual differences, followed by Affymetrix microarray to identify the signals involved in this embryo-maternal dialogue. Using laparoscopic insemination one oviduct of a sow was inseminated with spermatozoa and the contralateral oviduct was injected with diluent. This model allowed us to obtain samples from the oviduct and the tip of the uterine horn containing either embryos or oocytes from the same sow. Microarray analysis showed that most of the transcripts differentially expressed were down-regulated in the uterine horn in response to blastocysts when compared to oocytes. Many of the transcripts altered in response to the embryo in the uterine horn were related to the immune system. We used an in silico mathematical model to demonstrate the role of the embryo as a modulator of the immune system. This model revealed that relatively modest changes induced by the presence of the embryo could modulate the maternal immune response. These findings suggested that the presence of the embryo might regulate the immune system in the maternal tract to allow the refractory uterus to tolerate the embryo and support its development.
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影响因子：
2.7
作者：
Denker, HW
通讯作者：
Denker, HW
影响因子：
4.3
作者：
An GC;Faeder JR
通讯作者：
Faeder JR
影响因子：
2.8
作者：
Fazeli, A.
通讯作者：
Fazeli, A.
影响因子：
2.5
作者：
Chang, HS;Cheng, WTK;Choo, KB
通讯作者：
Choo, KB
影响因子：
6.1
作者：
Aboussahoud, Wedad;Bruce, Chris;Fazeli, Alireza
通讯作者：
Fazeli, Alireza