Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.
Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific.
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高产和低产湖羊卵巢的全基因组 DNA 甲基化和转录组分析
DOI：
10.3389/fcell.2022.820558
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发表时间：
2022
影响因子：
5.5
通讯作者：
Wang F
中科院分区：
文献类型：
--
作者：
Yao X;Li F;Wei Z;Ei-Samahy MA;Feng X;Yang F;Wang F
DNA 甲基化通过影响基因表达在生物过程中发挥重要作用。然而，DNA甲基化如何调节湖羊的表型变异仍不清楚。因此，我们在不同繁殖力和基因型（FecBB和FecB+）的湖羊卵巢中生成了全基因组DNA甲基化和转录组图谱。结果表明，高产湖羊和低产湖羊的卵巢DNA甲基化组和转录组存在显着差异。比较甲基化组分析分别鉴定了 10,644、9,594 和 12,214 个差异甲基化区域以及 87、1,121 和 2,375 个基因，显示三个不同比较组中的差异表达水平。富集了与雌性生殖相关的差异甲基化区域相关基因和差异表达基因，从而构建了各自的相互作用网络。此外，系统整合分析显示转录起始位点周围的DNA甲基化与基因表达水平之间存在负相关，这一点通过体内和体外测试整合素β2亚基（ITGB2）和溶酶体相关蛋白跨膜-4β（LAPTM4B）的表达得到证实。这些发现表明，DNA 甲基化通过影响卵巢中的基因表达来影响繁殖倾向，这可能有助于更好地了解表观基因组和转录组，从而有助于动物育种。
DNA methylation plays an important role in biological processes by affecting gene expression. However, how DNA methylation regulates phenotypic variation in Hu sheep remains unclear. Therefore, we generated genome-wide DNA methylation and transcriptomic profiles in the ovaries of Hu sheep with different prolificacies and genotypes (FecBB and FecB+). Results showed that ovary DNA methylome and transcriptome were significantly different between high prolificacy and low prolificacy Hu sheep. Comparative methylome analyses identified 10,644, 9,594, and 12,214 differentially methylated regions and 87, 1,121, and 2,375 genes, respectively, showing differential expression levels in three different comparison groups. Female reproduction-associated differentially methylated regions-related genes and differentially expressed genes were enriched, thereby the respective interaction networks were constructed. Furthermore, systematical integrative analyses revealed a negative correlation between DNA methylation around the transcriptional start site and gene expression levels, which was confirmed by testing the expression of integrin β2 subunit (ITGB2) and lysosome-associated protein transmembrane-4 beta (LAPTM4B) in vivo and in vitro. These findings demonstrated that DNA methylation influences the propensity for prolificacy by affecting gene expression in the ovaries, which may contribute to a greater understanding of the epigenome and transcriptome that will be useful for animal breeding.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.21521/mw.5601
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2016-12-01
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DOI：
10.1016/j.bbagrm.2020.194606
发表时间：
2020-09-01
影响因子：
4.7
作者：
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通讯作者：
Wang, Feng
DOI：
10.1111/j.1439-0388.1996.tb00627.x
发表时间：
1996-08-01
影响因子：
2.6
作者：
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Wassmuth, R