A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola.
A resistance locus in the American heirloom rice variety Carolina Gold Select is triggered by TAL effectors with diverse predicted targets and is effective against African strains of Xanthomonas oryzae pv. oryzicola.
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DOI：
10.1111/tpj.13212
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Leach JE
中科院分区：
文献类型：
--
作者：
Triplett LR;Cohen SP;Heffelfinger C;Schmidt CL;Huerta AI;Tekete C;Verdier V;Bogdanove AJ;Leach JE
水稻病原菌水稻黄单胞菌致病变种（pv.）稻瘟病菌和水稻条斑病菌产生大量类转录激活因子（TAL）效应物，这些效应物通过激活宿主感病基因的表达来增强细菌的致病性。水稻针对TAL效应物的抗性机制包括多态性，这种多态性可阻止效应物与感病基因启动子结合，或者允许效应物激活抗性基因。本研究在传统品种卡罗来纳黄金精选中发现了水稻抗性涉及TAL效应物的第三种机制。这种抗性表现为对来自两种水稻黄单胞菌致病变种的多种TAL效应物都有很强的病害发展抑制作用。这种抗性可由仅有3.5个中心重复单元的效应物触发，与决定TAL效应物结合特异性的重复可变双残基的组成无关，也与转录激活结构域无关。我们确定这种抗性由一个单一的显性位点赋予，该位点被命名为Xo1，它定位于4号染色体上的一个1.09 Mbp的片段。Xo1区间还对水稻条斑病菌非洲分支的菌株赋予完全抗性，代表了水稻中第一个针对细菌性条斑病的显性抗性位点。由Xo1赋予的TAL效应物触发的抗性与番茄抗性基因Bs4赋予的抗性在表型上的强烈相似性表明，单子叶植物和双子叶植物共享一种古老的或趋同进化的机制来识别类似的TAL效应物表位。
The rice pathogens Xanthomonas oryzae pathovar (pv.) oryzae and pv. oryzicola produce numerous transcription activator-like (TAL) effectors that increase bacterial virulence by activating expression of host susceptibility genes. Rice resistance mechanisms against TAL effectors include polymorphisms that prevent effector binding to susceptibility gene promoters, or that allow effector activation of resistance genes. This study identifies, in the heirloom variety Carolina Gold Select, a third mechanism of rice resistance involving TAL effectors. This resistance manifests through strong suppression of disease development in response to diverse TAL effectors from both X. oryzae pathovars. The resistance can be triggered by an effector with only 3.5 central repeats, is independent of the composition of the repeat variable diresidues that determine TAL effector binding specificity, and is independent of the transcriptional activation domain. We determined that the resistance is conferred by a single dominant locus, designated Xo1, that maps to a 1.09 Mbp fragment on chromosome 4. The Xo1 interval also confers complete resistance to the strains in the African clade of X. oryzae pv. oryzicola, representing the first dominant resistance locus against bacterial leaf streak in rice. The strong phenotypic similarity between the TAL effector triggered resistance conferred by Xo1 and that conferred by the tomato resistance gene Bs4 suggests that monocots and dicots share an ancient or convergently evolved mechanism to recognize analogous TAL effector epitopes.
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