Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.
Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.
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DOI：
10.1016/j.celrep.2021.109358
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发表时间：
2021-07-13
期刊：
影响因子：
8.8
通讯作者：
Cheng AG
中科院分区：
文献类型：
--
作者：
Jan TA;Eltawil Y;Ling AH;Chen L;Ellwanger DC;Heller S;Cheng AG
椭圆囊是前庭感觉器官，需要机械敏感毛细胞来检测线性加速度。在新生小鼠中，新的毛细胞来源于非感觉支持细胞，但细胞类型多样性和细胞添加机制的特征仍然很差。在这里，我们对单细胞转录组进行计算分析，对细胞类型进行分类，并分辨出14种感觉和非感觉亚型。沿着感觉上皮的外周，我们发现了不同的移行上皮细胞群，以Islr、Cnmd和Enpep表达为标志。通过重新构建从头轨迹和基因动力学，我们表明，随着椭圆囊的扩大，Islr+移行上皮细胞表现出动态和增殖阶段，以产生新的支持细胞，然后协调分化成毛细胞。总之，我们的研究揭示了一个顺序和协调的过程，非感觉上皮细胞有助于出生后小鼠感觉上皮的生长。出生后的小鼠椭圆囊扩大超过35%，毛细胞数量在前8天增加一倍。使用单细胞转录组学，Jan等人表明周围的移行上皮细胞增殖并通过逐步分化机制促进感觉上皮的扩张。
The utricle is a vestibular sensory organ that requires mechanosensitive hair cells to detect linear acceleration. In neonatal mice, new hair cells are derived from non-sensory supporting cells, yet cell type diversity and mechanisms of cell addition remain poorly characterized. Here, we perform computational analyses on single-cell transcriptomes to categorize cell types and resolve 14 individual sensory and non-sensory subtypes. Along the periphery of the sensory epithelium, we uncover distinct groups of transitional epithelial cells, marked by Islr, Cnmd, and Enpep expression. By reconstructing de novo trajectories and gene dynamics, we show that as the utricle expands, Islr+ transitional epithelial cells exhibit a dynamic and proliferative phase to generate new supporting cells, followed by coordinated differentiation into hair cells. Taken together, our study reveals a sequential and coordinated process by which non-sensory epithelial cells contribute to growth of the postnatal mouse sensory epithelium. The postnatal mouse utricle expands by more than 35% and doubles its number of hair cells during the first 8 days. Using single-cell transcriptomics, Jan et al. show that the surrounding transitional epithelial cells proliferate and contribute to the expansion of the sensory epithelium through a stepwise differentiation mechanism.
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影响因子：
16.6
作者：
Burns JC;Kelly MC;Hoa M;Morell RJ;Kelley MW
通讯作者：
Kelley MW
DOI：
10.1523/jneurosci.6274-11.2012
发表时间：
2012-05-09
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Burns JC;Cox BC;Thiede BR;Zuo J;Corwin JT
通讯作者：
Corwin JT
影响因子：
2.8
作者：
Freeman, S;Plotnik, M;Sohmer, H
通讯作者：
Sohmer, H
影响因子：
2.5
作者：
CORWIN, JT
通讯作者：
CORWIN, JT
影响因子：
25
作者：
Géléoc, GSG;Holt, JR
通讯作者：
Holt, JR