Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth.
Characterization of MtoD from Sideroxydans lithotrophicus: a cytochrome c electron shuttle used in lithoautotrophic growth.
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DOI：
10.3389/fmicb.2015.00332
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发表时间：
2015
影响因子：
5.2
通讯作者：
Clarke TA
中科院分区：
文献类型：
--
作者：
Beckwith CR;Edwards MJ;Lawes M;Shi L;Butt JN;Richardson DJ;Clarke TA
自养型氧化铁杆菌（Sideroxydans lithotrophicus）ES-1可以通过亚铁的氧化与氧的还原耦合而生长。可溶性亚铁在细胞表面被MtoAB孔蛋白-细胞色素复合物氧化，该复合物作为穿过外膜的电子导管。然后电子被运输到细胞质膜，在那里它们被用来产生质子动力（PMF）（用于ATP合成）和用于自养过程（如碳固定）的NADH。作为mtoAB基因簇的一部分，S.石养型还含有被提出编码细胞色素c蛋白的基因mtoD。我们从希瓦氏菌表达系统中分离出mtoD，其中mtoD基因在pBAD质粒载体上表达。纯化的MtoD的生物化学、生物物理学和晶体学表征显示其为含有单个血红素的11 kDa单体蛋白。序列和结构比对表明，MtoD属于1类细胞色素c家族，并具有类似的折叠铁细胞色素c552家族，但MtoD血红素是双组氨酸配位的，是显着更多的暴露比其他家庭成员的血红素。MtoD血红素在pH 7下的还原电位为+155 mV，与标准氢电极相比，比线粒体细胞色素c的还原电位低约100 mV。在从基因组中鉴定的潜在呼吸伴侣的背景下考虑MtoD的性质表明，MtoD可以与多个电子传递伴侣相关联，作为初级周质电子穿梭。
The autotrophic Sideroxydans lithotrophicus ES-1 can grow by coupling the oxidation of ferrous iron to the reduction of oxygen. Soluble ferrous iron is oxidized at the surface of the cell by an MtoAB porin-cytochrome complex that functions as an electron conduit through the outer membrane. Electrons are then transported to the cytoplasmic membrane where they are used to generate proton motive force (PMF) (for ATP synthesis) and NADH for autotrophic processes such as carbon fixation. As part of the mtoAB gene cluster, S. lithotrophicus also contains the gene mtoD that is proposed to encode a cytochrome c protein. We isolated mtoD from a Shewanella oneidensis expression system where the mtoD gene was expressed on a pBAD plasmid vector. Biochemical, biophysical, and crystallographic characterization of the purified MtoD revealed it as an 11 kDa monomeric protein containing a single heme. Sequence and structural alignment indicated that MtoD belonged to the class-1 cytochrome c family and had a similar fold to ferricytochrome c552 family, however the MtoD heme is bis-histidine coordinated and is substantially more exposed than the hemes of other family members. The reduction potential of the MtoD heme at pH 7 was +155 mV vs. Standard Hydrogen Electrode, which is approximately 100 mV lower than that of mitochondrial cytochrome c. Consideration of the properties of MtoD in the context of the potential respiratory partners identified from the genome suggests that MtoD could associate to multiple electron transfer partners as the primary periplasmic electron shuttle.
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影响因子：
4.4
作者：
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通讯作者：
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DOI：
10.1074/jbc.m112.348813
发表时间：
2012-04-20
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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影响因子：
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DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
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作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
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影响因子：
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