The effects of inhaled budesonide on circulating eosinophil progenitors and their expression of cytokines after allergen challenge in subjects with atopic asthma.
The effects of inhaled budesonide on circulating eosinophil progenitors and their expression of cytokines after allergen challenge in subjects with atopic asthma.
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吸入布地奈德对特应性哮喘受试者过敏原激发后循环嗜酸性粒细胞祖细胞及其细胞因子表达的影响。
DOI：
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发表时间：
2000
影响因子：
24.7
通讯作者：
P. O'Byrne
中科院分区：
文献类型：
--
作者：
G. Gauvreau;L. Wood;R. Sehmi;R. Watson;S. Dorman;R. Schleimer;J. Denburg;P. O'Byrne
异应性哮喘双应答者吸入过敏原与体外培养的Eo/B集落细胞中循环嗜酸性粒细胞/嗜碱性粒细胞集落形成单位（Eo/B CFU）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）免疫定位增加有关。目前的研究检测了吸入皮质类固醇布地奈德对过敏原诱导的循环嗜酸性粒细胞和Eo/B CFU数量的影响，以及体外培养的Eo/B集落细胞中GM-CSF和白细胞介素-5 （IL-5）的免疫定位。16名轻度特应性哮喘患者每天两次吸入200微克布地奈德或安慰剂，治疗7或8天。在吸入过敏原刺激前和刺激后24 h采集外周血，甲基纤维素培养非粘附性单核细胞（NAMC）。培养14 d后计数Eo/B CFU，并在载玻片上制备免疫细胞化学。布地奈德降低了过敏原诱导的循环嗜酸性粒细胞增加(4.0 +/- 0.4 × 10(5)/ml vs 6.5 +/- 0.7 × 10(5)/ml, p = 0.0001)，循环Eo/B CFU (12.4 +/- 2.3/10(6) NAMC vs 18.8 +/- 4.6/10(6) NAMC, p = 0.05)， Eo/B集落细胞中GM-CSF的免疫定位（11.8 +/- 1.9% vs 18.0 +/- 2.2%, p = 0.01），但IL-5的免疫定位（7.9 +/- 1.4% vs 4.5 +/- 0.6%, p > 0.05）。吸入布地奈德减少了GM-CSF存在下的变应原诱导的循环嗜酸性粒细胞及其祖细胞的数量，部分原因可能是调节了嗜酸性粒细胞祖细胞自分泌生长因子GM-CSF的表达。
Allergen inhalation by dual responder subjects with atopic asthma is associated with an increase in circulating eosinophil/basophil colony-forming units (Eo/B CFU) and granulocyte-macrophage colony- stimulating factor (GM-CSF) immunolocalization in Eo/B colony cells grown in vitro. The current study examined the effect of the inhaled corticosteroid, budesonide, on the number of allergen- induced circulating eosinophils and Eo/B CFU, and immunolocalization of GM-CSF and interleukin-5 (IL-5) in Eo/B colony cells grown in vitro. Sixteen subjects with mild atopic asthma were treated for either 7 or 8 d with 200 microg inhaled budesonide or placebo twice a day. Peripheral blood was collected before and 24 h after allergen inhalation challenge and nonadherent mononuclear cells (NAMC) were grown in methylcellulose culture. Eo/B CFU were enumerated after 14 d in culture, and prepared on slides for immunocytochemistry. Budesonide attenuated the allergen-induced increase in circulating eosinophils (4.0 +/- 0.4 x 10(5)/ml versus 6.5 +/- 0.7 x 10(5)/ml, p = 0.0001), circulating Eo/B CFU (12.4 +/- 2.3/10(6) NAMC versus 18.8 +/- 4.6/10(6) NAMC, p = 0.05), and immunolocalization of GM-CSF in Eo/B colony cells (11.8 +/- 1.9% positive versus 18.0 +/- 2.2%, p = 0.01) but not immunolocalization of IL-5 (7.9 +/- 1.4% versus 4.5 +/- 0.6%, p > 0.05). Inhaled budesonide attenuated the number of allergen-induced circulating eosinophils and their progenitors grown in the presence of GM-CSF, which may partially be a result of regulating eosinophil progenitor expression of the autocrine growth factor GM-CSF.
影响因子：
14.2
作者：
Lilly, CM;Woodruff, PG;Hanrahan, JP
通讯作者：
Hanrahan, JP
影响因子：
14.2
作者：
BROIDE, DH;LOTZ, M;WASSERMAN, SI
通讯作者：
WASSERMAN, SI
通过培养的人气管上皮细胞产生粒细胞-巨噬细胞集落刺激因子。
影响因子：
6.4
作者：
Churchill,L;Friedman,B;Schleimer,RP;Proud,D
通讯作者：
Proud,D