Cinnamaldehyde regulates the synthesis of Alternaria alternata non-host selective toxins by influencing PKS gene expression and oxidoreductase activity
Cinnamaldehyde regulates the synthesis of Alternaria alternata non-host selective toxins by influencing PKS gene expression and oxidoreductase activity
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肉桂醛通过影响PKS基因表达和氧化还原酶活性调节链格孢非宿主选择性毒素的合成
DOI：
10.1016/j.indcrop.2019.112074
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发表时间：
2020-03
影响因子：
5.9
通讯作者：
Jing Guoxing
中科院分区：
文献类型：
--
作者：
Liu Miao;Jing Guoxing
链格孢菌是引起果蔬黑腐病的病原菌，在病原菌入侵过程中产生大量有害代谢产物。本研究表明，亚抑制浓度0.0125 μl/mL的肉桂醛不能抑制A.在马铃薯葡萄糖肉汤（PDB）中，非寄主选择性毒素（NHSTs）的产生明显减少。转录组数据显示，肉桂醛处理后差异表达基因（DEG）中以下调基因为主，且大多数DEG分布在代谢途径和次生代谢产物的生物合成中。通过转录组测序，筛选出与真菌毒素生物合成相关的4个聚酮酶基因（PksI、PksJ、PksFan和PksA），肉桂醛孵育可显著下调其表达量。此外，qRT-PCR结果表明，肉桂醛抑制了A.另外，肉桂醛处理降低了丙二醛（MDA）含量，提高了过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性，降低了活性氧（ROS）水平，说明肉桂醛可以提高抗氧化酶活性，消除过量ROS的产生。结果表明，肉桂醛可能通过活性氧信号转导介导了链格孢酚（AOH）、链格孢酚单甲醚（AME）和细交链孢酮酸（TEA）的合成。交替
Alternaria alternatacauses Black Rot disease in fruits and vegetables, and could produce numerous harmful metabolites during pathogen invasion. This study demonstrated that a sub-inhibitory concentration of 0.0125 μl/mL cinnamaldehyde could not inhibit the mycelial growth ofA. alternatain Potato dextrose broth (PDB), but the production of non-host selective toxins (NHSTs) was obviously reduced after incubation. The transcriptome data showed that down-regulated genes dominated the differentially expressed genes (DEGs) after cinnamaldehyde treatment, and most DEGs were distributed in metabolic pathways and biosynthesis of secondary metabolites. Four polyketide synthases genes (PksI,PksJ,PksFandPksA) related to mycotoxin biosynthesis in secondary metabolites were screened out after transcriptome sequencing and the expression quantity was significantly down-regulated by cinnamaldehyde incubation. Moreover, the qRT-PCR results showed that cinnamaldehyde inhibited the expression of 4Pksgenes during the growth ofA. alternata.In addition, cinnamaldehyde decreased the malondialdehyde (MDA) content but increased the enzymes activities of catalase (CAT) and superoxide dismutase (SOD), the lower reactive oxygen species (ROS) level in the cinnamaldehyde-treated group demonstrated that cinnamaldehyde could improve the antioxidase activities and eliminate excessive ROS production. The results showed that cinnamaldehyde might, through ROS signal transduction, mediate the mycotoxin synthesis of alternariol (AOH), alternariol monomethyl ether (AME) and tenuazonic acid (TEA) inA. alternata.
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影响因子：
7
作者：
Li, Jiangkuo;Li, Hua;Qin, Guozheng
通讯作者：
Qin, Guozheng
影响因子：
5.2
作者：
Fountain JC;Bajaj P;Nayak SN;Yang L;Pandey MK;Kumar V;Jayale AS;Chitikineni A;Lee RD;Kemerait RC;Varshney RK;Guo B
通讯作者：
Guo B
影响因子：
4.9
作者：
Shuzhi Yuan;Jiaqi Yan;Meng Wang;Xinyuan Ding;Yinan Zhang;Wusun Li;Jiankang Cao;Weibo Jiang
通讯作者：
Shuzhi Yuan;Jiaqi Yan;Meng Wang;Xinyuan Ding;Yinan Zhang;Wusun Li;Jiankang Cao;Weibo Jiang
影响因子：
5.9
作者：
Lingchun Xu;T. Neng-guo;Yang Wenhao;Guoxing Jing
通讯作者：
Lingchun Xu;T. Neng-guo;Yang Wenhao;Guoxing Jing
影响因子：
7.4
作者：
Jayashree, T;Subramanyam, C
通讯作者：
Subramanyam, C