Affinity adsorption mechanism studies of adsorbents C1-Zn(II) for uremic middle molecular peptides containing Asp-Phe-Leu-Ala-Glu sequence
Affinity adsorption mechanism studies of adsorbents C1-Zn(II) for uremic middle molecular peptides containing Asp-Phe-Leu-Ala-Glu sequence
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吸附剂C1-Zn(II)对含Asp-Phe-Leu-Ala-Glu序列尿毒症中分子肽的亲和吸附机理研究
DOI：
10.1007/s11426-010-4193-7
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
Yuan Zhi
中科院分区：
文献类型：
--
作者：
Li PingLin;Fu LiXue;Qiao YiTao;Zhao JianXin;Wang Wei;Yuan Zhi
为开发血液灌流法去除含有DFLAE（DE 5，尿毒症血清中积累的典型肽序列）的中分子肽的高效吸附剂，设计并合成了3种对DE 5具有高亲和性的亲和吸附剂（C1-Zn 2+、C2-Zn 2+和C3-Zn 2+）。随后，我们通过静态吸附实验和等温滴定量热法（ITC）评价了每种吸附剂的相应吸附能力。结果表明，C1-Zn 2+对含DE 5多肽的吸附能力最强，吸附容量为8.52 mg/g。通过改变吸附条件，阐明了吸附机理。吸附的主要驱动力是金属-羧基配位作用，疏水力起协同作用。我们的工作可以为设计对寡肽具有高亲和性和选择性的吸附剂提供基础知识。
To exploit efficient adsorbents for removing middle molecular peptides containing DFLAE (DE5, a typical peptide sequence accumulated in uremic serum) sequence by hemoperfusion, we designed and synthesized three affinity adsorbents (C1-Zn2+, C2-Zn2+ and C3-Zn2+) that could have high affinity to DE5. Subsequently, we evaluated the corresponding adsorption ability of each adsorbent by static adsorption experiments and isothermal titration calorimetry (ITC). The results showed that C1-Zn2+ had the best adsorption ability to DE5-containing peptides and the adsorption capacity for DE5 was 8.52 mg/g. By changing the adsorption conditions, the adsorption mechanism was elucidated. The main driving force of the adsorption is metal-carboxyl coordination and the hydrophobic force affords the cooperative effect. It is expected that our present work can provide basic understanding for the design of adsorbents with high affinity and selectivity towards oligopeptides.
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影响因子：
9.6
作者：
Chu Jiegen;Xiaohang Liu;Zhi Yuan;B. He
通讯作者：
Chu Jiegen;Xiaohang Liu;Zhi Yuan;B. He
影响因子：
5.4
作者：
Jianxin Zhao;Yitao Qiao;Jing Feng;Zhi Yuan
通讯作者：
Jianxin Zhao;Yitao Qiao;Jing Feng;Zhi Yuan
DOI：
10.1016/j.jchromb.2003.08.008
发表时间：
2003-10
期刊：
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
影响因子：
--
作者：
B. Kaplan;M. Cojocaru;E. Unsworth;A. Knecht;B. Martin
通讯作者：
B. Kaplan;M. Cojocaru;E. Unsworth;A. Knecht;B. Martin
影响因子：
14
作者：
Zhi Yuan;Mei Yu;Jihong Li;Guanghui Hou;Huiyan Wang
通讯作者：
Zhi Yuan;Mei Yu;Jihong Li;Guanghui Hou;Huiyan Wang
影响因子：
6.2
作者：
Guohua Li;Jihong Li;Wei Wang;Mei Yang;Yuanwei Zhang;Pingchuan Sun;Zhi Yuan;B. He;Yao-ting Yu
通讯作者：
Guohua Li;Jihong Li;Wei Wang;Mei Yang;Yuanwei Zhang;Pingchuan Sun;Zhi Yuan;B. He;Yao-ting Yu