Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization
Magnetic combined cross-linked enzyme aggregates of horseradish peroxidase and glucose oxidase: an efficient biocatalyst for dye decolourization
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辣根过氧化物酶和葡萄糖氧化酶的磁性组合交联酶聚集体：一种高效的染料脱色生物催化剂
DOI：
10.1039/c6ra12009a
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发表时间：
2016-09
期刊：
影响因子：
3.9
通讯作者：
Jing Gao
中科院分区：
文献类型：
--
作者：
Yanjun Jiang;Li Ma;Ying He;Jing Gao
本研究设计并成功制备了葡萄糖氧化酶（GOD）和辣根过氧化物酶（HRP）的磁性复合交联酶聚集体（combi-CLEAs）。采用共沉淀法和交联法制备了磁性复合型CLEAs，并将GOD和HRP固定在氨基功能化的Fe 3 O 4粒子上。乙醇和戊二醛分别用作沉淀剂和交联剂。将磁性复合型CLEAs应用于直接黑38（DB 38）的去除，考察了磁性复合型CLEAs的催化性能。结果表明，磁性复合CLEA在pH 6.0和40 °C下对DB 38具有较高的去除效率。在最佳条件下，DB 38的最高去除率为92.28%，高于游离酶的46.82%。重复使用8次后，DB 38去除率仍能达到55.53%，说明磁性复合CLEA具有良好的重复使用性。由于易于分离和高重复使用性，本研究表明，磁性复合CLEAs可能是一种环境友好和成本效益的生物催化剂，用于染料去除和水处理。
In this study, the magnetic combined cross-linked enzyme aggregates (combi-CLEAs) of glucose oxidase (GOD) and horseradish peroxidase (HRP) were designed and prepared successfully. The magnetic combi-CLEAs were fabricated by co-precipitation and cross-linking for the immobilization of GOD and HRP onto amino-functionalized Fe3O4 particles. Ethanol and glutaraldehyde were used as precipitant and cross-linker, respectively. In order to evaluate the catalytic performance, the magnetic combi-CLEAs were applied in the removal of direct black-38 (DB38). The results indicated that the magnetic combi-CLEAs exhibited high removal efficiency for DB38 at pH 6.0 and 40 °C. Under optimal conditions, the highest removal efficiency of DB38 was 92.28%, which was higher than that of free enzymes (46.82%). After 8 cycles of reuse, the DB38 removal efficiency could still reach 55.53%, indicating that the magnetic combi-CLEAs had good reusability. Due to the easy separation and high reusability, this study shows that magnetic combi-CLEAs may potentially be an environmentally friendly and cost effective biocatalyst for dye removal and water treatments.
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