Screening and identification of key biomarkers in hepatocellular carcinoma: Evidence from bioinformatic analysis.
Screening and identification of key biomarkers in hepatocellular carcinoma: Evidence from bioinformatic analysis.
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肝细胞癌中关键生物标志物的筛查和鉴定：来自生物信息学分析的证据。
DOI：
10.3892/or.2017.5946
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发表时间：
2017-11
期刊：
影响因子：
4.2
通讯作者：
Qin B
中科院分区：
文献类型：
--
作者：
Li L;Lei Q;Zhang S;Kong L;Qin B
肝细胞癌（HCC）是世界范围内最常见的癌症之一。尽管人们对肝癌的发病机制进行了大量的研究，但其分子机制仍不十分清楚。为筛选肝癌发生发展的候选基因，从GEO数据库下载基因表达谱芯片数据集GSE 19665、GSE 33006和GSE 41804。鉴定差异表达基因（DEG），并进行功能富集分析。构建了蛋白质相互作用网络（PPI），并利用STRING和Cytoscape进行了模块分析。共鉴定出273个DEG，包括189个下调基因和84个上调基因。DEG丰富的功能和途径包括蛋白质激活级联、补体激活、碳水化合物结合、补体和凝血级联、有丝分裂细胞周期和卵母细胞减数分裂。通过生物学过程分析发现，这些基因主要富集在细胞分裂、细胞周期和核分裂过程中。生存分析显示BUB 1、CDC 20、KIF 20 A、RACGAP 1和CEP 55可能参与HCC的发生、侵袭或复发。总之，本研究中发现的DEGs和hub基因有助于我们了解HCC发生和发展的分子机制，并为HCC的诊断和治疗提供候选靶点。
Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide. Intense efforts have been made to elucidate the pathogeny, but the molecular mechanisms of HCC are still not well understood. To identify the candidate genes in the carcinogenesis and progression of HCC, microarray datasets GSE19665, GSE33006 and GSE41804 were downloaded from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified, and function enrichment analyses were performed. The protein-protein interaction network (PPI) was constructed and the module analysis was performed using STRING and Cytoscape. A total of 273 DEGs were identified, consisting of 189 downregulated genes and 84 upregulated genes. The enriched functions and pathways of the DEGs include protein activation cascade, complement activation, carbohydrate binding, complement and coagulation cascades, mitotic cell cycle and oocyte meiosis. Sixteen hub genes were identified and biological process analysis revealed that these genes were mainly enriched in cell division, cell cycle and nuclear division. Survival analysis showed that BUB1, CDC20, KIF20A, RACGAP1 and CEP55 may be involved in the carcinogenesis, invasion or recurrence of HCC. In conclusion, DEGs and hub genes identified in the present study help us understand the molecular mechanisms underlying the carcinogenesis and progression of HCC, and provide candidate targets for diagnosis and treatment of HCC.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
24.5
作者：
Chen J;Rajasekaran M;Xia H;Zhang X;Kong SN;Sekar K;Seshachalam VP;Deivasigamani A;Goh BK;Ooi LL;Hong W;Hui KM
通讯作者：
Hui KM
影响因子：
1.6
作者：
Franklin, Renty B;Levy, Bernard A;Zou, Jing;Hanna, Nader;Desouki, Mohamed Mokhtar;Bagasra, Omar;Johnson, Leslie A;Costello, Leslie C
通讯作者：
Costello, Leslie C
影响因子：
3.7
作者：
Elgaaen, Bente Vilming;Olstad, Ole Kristoffer;Gautvik, Kaare M.
通讯作者：
Gautvik, Kaare M.
影响因子：
3.3
作者：
Chen, X;Cheung, ST;Brown, PO
通讯作者：
Brown, PO