Augmented plasma microparticles during acute Plasmodium vivax infection.
Augmented plasma microparticles during acute Plasmodium vivax infection.
复制标题
DOI：
10.1186/1475-2875-9-327
复制
发表时间：
2010-11-16
期刊：
影响因子：
3
通讯作者：
Carvalho LH
中科院分区：
文献类型：
--
作者：
Campos FM;Franklin BS;Teixeira-Carvalho A;Filho AL;de Paula SC;Fontes CJ;Brito CF;Carvalho LH
在过去的几年中，微粒（MP）-激活或凋亡时从细胞释放的亚微米囊泡-的研究在炎症和感染性疾病领域获得了越来越多的兴趣。它们在人类疟疾寄生虫间日疟原虫中的作用尚未探索。由于急性间日疟与促炎反应有关，本研究中研究的主要假设是间日疟原虫感染与循环MP水平升高相关，这可能在非免疫患者的急性疾病中发挥作用。对来自巴西亚马逊地区疟疾传播不稳定地区的37例无并发症间日疟原虫感染者的血浆MP进行了分析。使用经典的MP标记物、膜联蛋白和针对特定细胞表面标记物的荧光染料标记的单克隆抗体通过流式细胞术分析MP表型。将间日疟原虫患者（n = 37）的血浆MP频率进一步与未暴露于疟疾的对照（n = 15）和卵巢癌患者（n = 12）（一种已知的与疟疾无关的MP诱导疾病）进行比较。与年龄匹配的未暴露于疟疾的健康对照组相比，间日疟原虫患者的血浆循环MP频率显著增加。虽然血小板、红细胞和白细胞是间日疟期间MP的主要细胞来源，但血小板衍生的MP（PMPs）在采血时（β = 0.06，p < 0.0001）和急性症状持续时间（β = 0.36，p < 0.0001）的存在下以线性方式增加。最后，结果表明，随着患者经历更多的临床疟疾发作，血浆PMPs水平降低（β = 0.07，p < 0.003）。间日疟原虫急性感染时存在丰富的循环MP，血小板源性MP可能在间日疟急性炎症症状中发挥作用。
In the last few years, the study of microparticles (MPs) - submicron vesicles released from cells upon activation or apoptosis - has gained growing interest in the field of inflammation and in infectious diseases. Their role in the human malaria parasite Plasmodium vivax remains unexplored. Because acute vivax malaria has been related to pro-inflammatory responses, the main hypothesis investigated in this study was that Plasmodium vivax infection is associated with elevated levels of circulating MPs, which may play a role during acute disease in non-immune patients. Plasma MPs were analysed among thirty-seven uncomplicated P. vivax infections from an area of unstable malaria transmission in the Brazilian Amazon. The MP phenotype was analysed by flow cytometry using the classical MP marker, annexin, and fluorochrome-labeled monoclonal antibodies against specific cell surface markers. The frequencies of plasma MPs in P. vivax patients (n = 37) were further compared to malaria-unexposed controls (n = 15) and ovarian carcinoma patients (n = 12), a known MPs-inducing disease non-related to malaria. The frequencies of plasma circulating MPs were markedly increased in P. vivax patients, as compared to healthy age-matched malaria-unexposed controls. Although platelets, erythrocytes and leukocytes were the main cellular sources of MPs during vivax malaria, platelet derived-MPs (PMPs) increased in a linear fashion with the presence of fever at the time of blood collection (β = 0.06, p < 0.0001) and length of acute symptoms (β = 0.36, p < 0.0001). Finally, the results suggest that plasma levels of PMPs diminish as patient experience more episodes of clinical malaria (β = 0.07, p < 0.003). Abundant circulating MPs are present during acute P. vivax infection, and platelet derived-MPs may play a role on the acute inflammatory symptoms of malaria vivax.
登录
查看更多内容
DOI：
10.1086/510756
发表时间：
2007-02-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Anstey NM;Handojo T;Pain MC;Kenangalem E;Tjitra E;Price RN;Maguire GP
通讯作者：
Maguire GP
影响因子：
6.4
作者：
Carvalho, Bruna O.;Lopes, Stefanie C. P.;Costa, Fabio T. M.
通讯作者：
Costa, Fabio T. M.
影响因子：
4.8
作者：
Faille, Dorothee;Combes, Valery;Grau, Georges E.
通讯作者：
Grau, Georges E.
疟原虫疟原虫：阵发性相关的脂质介导白细胞聚集。
影响因子：
3
作者：
Karunaweera, Nadira;Wanasekara, Deepani;Chandrasekharan, Vishvanath;Mendis, Kamini;Carter, Richard
通讯作者：
Carter, Richard
DOI：
10.1073/pnas.89.8.3200
发表时间：
1992-04-15
影响因子：
11.1
作者：
KARUNAWEERA, ND;GRAU, GE;MENDIS, KN
通讯作者：
MENDIS, KN