Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast
Cdc13 (cyclin B) is degraded by autophagy under sulfur depletion in fission yeast
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Cdc13（细胞周期蛋白 B）在裂殖酵母中硫耗尽时被自噬降解
DOI：
10.1080/27694127.2022.2047442
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Aiba Hirofumi
中科院分区：
文献类型：
--
作者：
Ohtsuka Hokuto;Hatta Yoshiko;Hayashi Kana;Shimasaki Takafumi;Otsubo Yoko;Ito Yurika;Tsutsui Yu;Hattori Nobutake;Yamashita Akira;Murakami Hiroshi;Aiba Hirofumi
在正常有丝分裂中，细胞周期蛋白被后期促进复合体/环小体（APC/C）介导的蛋白酶体降解。我们发现Cdc13（细胞周期蛋白B）在缺硫裂变酵母中也被巨噬/自噬降解。硫耗竭导致g2细胞周期停滞，细胞体积减小；然而，相关机制尚不清楚。我们发现自噬是Cdc13降解所必需的，这与细胞周期阻滞和细胞大小减小有关，通过检查硫耗尽下的细胞形态。Cdc13- gfp融合蛋白的分析支持Cdc13通过自噬降解的结论。此外，我们还发现硫缺失会通过ecl1家族蛋白导致雷帕霉素复合物1靶点（TORC1）活性失活。我们的数据表明，细胞周期蛋白被两个不同的系统降解：APC/ c介导的蛋白酶体和自噬。后者是在营养耗尽的情况下诱发的。当资源耗尽时，降解系统中的这种开关将有助于适当的细胞周期停止。缩写：APC，后期促进复合体；细胞周期蛋白依赖性激酶；DB，破坏箱；EMM，爱丁堡最小培养基；绿色荧光蛋白；聚合酶链式反应；TOR，雷帕霉素靶；UPS，泛素-蛋白酶体系统
Cyclins are degraded by the anaphase-promoting complex/cyclosome (APC/C)-mediated proteasome in normal mitosis. We showed that Cdc13 (cyclin B) is also degraded by macroautophagy/autophagy in sulfur-deficient fission yeast. Sulfur depletion causes G2cell cycle arrest and reduces cell size; however, the associated mechanisms are unknown. We found that autophagy is required for the degradation of Cdc13, which is associated with cell cycle arrest and reduced cell size, by examining cell morphology under sulfur depletion. The analysis of the Cdc13-GFP fusion protein supported the conclusion that Cdc13 is degraded by autophagy. Moreover, we showed that sulfur depletion results in the inactivation of target of rapamycin complex 1 (TORC1) activity via Ecl1-family proteins. Our data indicate that the cyclin is degraded by two different systems: APC/C-mediated proteasome and autophagy. The latter is induced under nutrient-depleted situations. This switch in degradation systems will contribute to appropriate cell cycle arrest when resources are depleted.Abbreviations:APC, anaphase-promoting complex; CDK, cyclin-dependent kinase; DB, destruction box; EMM, Edinburgh minimal medium; GFP, green fluorescent protein; PCR, polymerase chain reaction; TOR, target of rapamycin; UPS, ubiquitin-proteasome system
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影响因子：
2.6
作者：
Sato, M;Dhut, S;Toda, T
通讯作者：
Toda, T
影响因子：
3.1
作者：
Hokuto Ohtsuka;Takanori Kato;Teppei Sato;Takafumi Shimasaki;Takaaki Kojima;H. Aiba
通讯作者：
Hokuto Ohtsuka;Takanori Kato;Teppei Sato;Takafumi Shimasaki;Takaaki Kojima;H. Aiba
影响因子：
3.7
作者：
Caspari T;Hilditch V
通讯作者：
Hilditch V
WD 重复蛋白通过负向调节 Cdc2/B 型细胞周期蛋白复合物来控制细胞周期和分化。
影响因子：
3.3
作者：
Satoko Yamaguchi;H. Murakami;Hiroshi Murakami;Hiroto Okayama
通讯作者：
Hiroto Okayama
裂殖酵母葡聚糖形成和生长之间的协调需要鞘脂酶活性。
影响因子：
3.3
作者：
Feoktistova,A;Magnelli,P;Abeijon,C;Perez,P;Lester,RL;Dickson,RC;Gould,KL
通讯作者：
Gould,KL