Insights into the Differential Preservation of Bone Proteomes in Inhumed and Entombed Cadavers from Italian Forensic Caseworks.
Insights into the Differential Preservation of Bone Proteomes in Inhumed and Entombed Cadavers from Italian Forensic Caseworks.
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DOI：
10.1021/acs.jproteome.1c00904
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发表时间：
2022-05-06
影响因子：
4.4
通讯作者：
Procopio, Noemi
中科院分区：
文献类型：
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作者：
Bonicelli, Andrea;Di Nunzio, Aldo;Di Nunzio, Ciro;Procopio, Noemi
关键词：
骨骼是一种坚硬的生物组织，也是法医调查中宝贵的信息库，因为它保留了通常用于识别目的的关键生物分子。骨蛋白最近因其在估计死后间隔 (PMI) 和死亡年龄 (AAD) 方面的潜力而引起了人们的极大兴趣。然而，此类蛋白质的保存高度依赖于内在和外在因素，这些因素可能阻碍分子技术在法医学中的潜在应用。本研究旨在调查两种常用的埋葬方式（埋葬和土葬）对骨蛋白存活的影响。该样本由意大利南部墓地中挖掘出的 14 名个体组成，他们具有不同的 AAD（29-85 岁）和 PMI（1-37 岁）。 LC-MS/MS 分析表明，16 种蛋白质在埋藏条件下得到更好的保存，4 种蛋白质在土埋条件下得到更好的保存，而翻译后蛋白质修饰没有检测到明显差异。此外，还确定了一些潜在的“稳定”蛋白质标记（即不受埋藏环境影响的蛋白质），用于 PMI 和 AAD 估计。总的来说，这些结果表明，两种埋葬环境在非胶原蛋白的差异保存中发挥着作用，证实了基于 LC-MS/MS 的蛋白质组学在法医学中的潜力。
Bone is a hard biological tissue and a precious reservoir of information in forensic investigations as it retains key biomolecules commonly used for identification purposes. Bone proteins have recently attracted significant interest for their potential in estimating post-mortem interval (PMI) and age at death (AAD). However, the preservation of such proteins is highly dependent on intrinsic and extrinsic factors that can hinder the potential application of molecular techniques to forensic sciences. The present study aims at investigating the effects that two commonly used types of burial practices (entombment and inhumation) have on bone protein survival. The sample consists of 14 exhumed individuals from cemeteries in Southern Italy with different AADs (29–85 years) and PMIs (1–37 years). LC-MS/MS analyses show that 16 proteins are better preserved under the entombed conditions and 4 proteins are better preserved under the inhumed conditions, whereas no clear differences are detected for post-translational protein modifications. Furthermore, several potential “stable” protein markers (i.e., proteins not affected by the burial environment) are identified for PMI and AAD estimation. Overall, these results show that the two burial environments play a role in the differential preservation of noncollagenous proteins, confirming the potential of LC-MS/MS-based proteomics in forensic sciences.
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DOI：
10.1039/c4ay02943g
发表时间：
2015-04-07
期刊：
Analytical methods : advancing methods and applications
影响因子：
--
作者：
Longato S;Wöss C;Hatzer-Grubwieser P;Bauer C;Parson W;Unterberger SH;Kuhn V;Pemberger N;Pallua AK;Recheis W;Lackner R;Stalder R;Pallua JD
通讯作者：
Pallua JD
影响因子：
2.1
作者：
Hyde, Embriette R.;Haarmann, Daniel P.;Bucheli, Sibyl R.
通讯作者：
Bucheli, Sibyl R.
影响因子：
1.6
作者：
Booth, T. J.
通讯作者：
Booth, T. J.
影响因子：
4.1
作者：
Bonnet, Nicolas;Garnero, Patrick;Ferrari, Serge
通讯作者：
Ferrari, Serge
影响因子：
2.2
作者：
Forbes, SL;Stuart, BH;Dent, BB
通讯作者：
Dent, BB