PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana.
PtrWRKY73, a salicylic acid-inducible poplar WRKY transcription factor, is involved in disease resistance in Arabidopsis thaliana.
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PtrWRKY73 是一种水杨酸诱导的杨 WRKY 转录因子，参与拟南芥的抗病性。
DOI：
10.1007/s00299-015-1745-5
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发表时间：
2015-05
影响因子：
6.2
通讯作者：
Luo K
中科院分区：
文献类型：
--
作者：
Duan Y;Jiang Y;Ye S;Karim A;Ling Z;He Y;Yang S;Luo K
从毛果杨树中分离并鉴定了一个水杨酸诱导的WRKY基因PtrWRKY73。PtrWRKY73在拟南芥中的过表达提高了对生物营养型病原菌的抗性，但降低了对坏死型病原菌的抗性。WRKY转录因子通常参与植物的防御反应。然而，关于WRKY基因在杨树防御中的作用的信息有限。在本研究中，我们从毛果杨中分离到一个水杨酸(SA)诱导的WRKY基因PtrWRKY73，它属于组I家族，包含两个WRKY结构域，一个D结构域和一个SP簇。PtrWRKY73主要在根、老叶、芽和茎中表达，尤其是韧皮部，其表达受外源SA诱导。PtrWRKY73定位于植物细胞核，具有转录激活作用。PtrWRKY73在拟南芥中的过表达提高了对细菌致病菌株丁香假单胞菌(PstDC3000)的抗性，但对坏死性真菌灰霉病菌(Botrytis Cinerea)的敏感性增加。在过量表达PtrWRKY73的转基因植株中，SA介导的防御相关基因PR1、PR2和PAD4显著上调。在PtrWRKY73高表达植株中，PR1的非表达基因(NPR1)没有受到影响，而与防御相关的基因PAL4却减少了。这些结果表明，PtrWRKY73在植物对生物营养性病原菌的抗性中起着积极的作用，但对植物对坏死性病原菌的抗性具有负面影响。本文的在线版本(doi：10.1007/s00299-0151745-5)包含补充材料，授权用户可以使用。
A salicylic acid-inducible WRKY gene, PtrWRKY73, from Populus trichocarpa , was isolated and characterized. Overexpression of PtrWRKY73 in Arabidopsis thaliana increased resistance to biotrophic pathogens but reduced resistance against necrotrophic pathogens. WRKY transcription factors are commonly involved in plant defense responses. However, limited information is available about the roles of the WRKY genes in poplar defense. In this study, we isolated a salicylic acid (SA)-inducible WRKY gene, PtrWRKY73, from Populus trichocarpa, belonging to group I family and containing two WRKY domains, a D domain and an SP cluster. PtrWRKY73 was expressed predominantly in roots, old leaves, sprouts and stems, especially in phloem and its expression was induced in response to treatment with exogenous SA. PtrWRKY73 was localized to the nucleus of plant cells and exhibited transcriptional activation. Overexpression of PtrWRKY73 in Arabidopsis thaliana resulted in increased resistance to a virulent strain of the bacterial pathogen Pseudomonas syringae (PstDC3000), but more sensitivity to the necrotrophic fungal pathogen Botrytis cinerea. The SA-mediated defense-associated genes, such as PR1, PR2 and PAD4, were markedly up-regulated in transgenic plants overexpressing PtrWRKY73. Arabidopsis non-expressor of PR1 (NPR1) was not affected, whereas a defense-related gene PAL4 had reduced in PtrWRKY73 overexpressor plants. Together, these results indicated that PtrWRKY73 plays a positive role in plant resistance to biotrophic pathogens but a negative effect on resistance against necrotrophic pathogens. The online version of this article (doi:10.1007/s00299-015-1745-5) contains supplementary material, which is available to authorized users.
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影响因子：
7.2
作者：
Kloek, AP;Verbsky, ML;Kunkel, BN
通讯作者：
Kunkel, BN
影响因子：
6.9
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Encinas-Villarejo, Sonia;Maldonado, Ana M.;Caballero, Jose L.
通讯作者：
Caballero, Jose L.
影响因子：
56.9
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F