Genome-Wide Identification and Expression Analysis of the Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Gene Families in Rose (Rosa hybrida cv 'Carola') under Different Light Conditions.
Genome-Wide Identification and Expression Analysis of the Trehalose-6-phosphate Synthase and Trehalose-6-phosphate Phosphatase Gene Families in Rose (Rosa hybrida cv 'Carola') under Different Light Conditions.
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DOI：
10.3390/plants13010114
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发表时间：
2023-12-31
期刊：
影响因子：
--
通讯作者：
Che D
中科院分区：
文献类型：
--
作者：
Fan Y;Gao P;Zhou T;Pang S;Zhang J;Yang T;Zhang W;Dong J;Che D
海藻糖、海藻糖-6-磷酸合成酶（TPS）和海藻糖-6-磷酸酶（TPP）在植物非生物胁迫和生长发育中起重要作用。然而，它们在蔷薇开花过程中的作用尚未明确。本研究发现，在较短的光周期或较弱的光强下，月季（Rosa hybrida cv ' Carola '）茎尖分生组织中海藻糖含量显著降低，导致开花时间延迟。在基因组中共鉴定出9个RhTPPs基因和7个RhTPPs基因。顺式元件分析表明，RhTPS和RhTPP基因与植物激素和环境胁迫反应有关。转录组数据分析显示，rhtps和RhTPPs家族基因在不同组织中的表达水平存在显著差异，表明RhTPPF和RhTPPJ是参与不同光环境下玫瑰花蕾发育的潜在关键基因。定量实时反转录（qRT-PCR）结果进一步表明，在短光周期和弱光强度下，RhTPP所有成员均显著下调。RhTPS1a、RhTPS10和RhTPS11在短光周期下表达量上调，且与开花时间和海藻糖含量降低呈负相关。在弱光强下，RhTPS11表达上调和负调控开花，RhTPS5、RhTPS6、RhTPS7b、RhTPS9和RhTPS10表达下调和正调控开花。本研究为揭示RhTPS和RhTPP基因家族在玫瑰海藻糖调控中的作用奠定了基础。
Trehalose, trehalose-6-phosphate synthase (TPS),and trehalose-6-phosphatase (TPP) have been reported to play important roles in plant abiotic stress and growth development. However, their functions in the flowering process of Rosa hybrida have not been characterized. In this study we found that, under a short photoperiod or weak light intensity, the content of trehalose in the shoot apical meristem of Rosa hybrida cv ‘Carola’ significantly decreased, leading to delayed flowering time. A total of nine RhTPSs and seven RhTPPs genes were identified in the genome. Cis-element analysis suggested that RhTPS and RhTPP genes were involved in plant hormones and environmental stress responses. Transcriptome data analysis reveals significant differences in the expression levels of RhTPSs and RhTPPs family genes in different tissues and indicates that RhTPPF and RhTPPJ are potential key genes involved in rose flower bud development under different light environments. The results of quantitative real-time reverse transcription (qRT-PCR) further indicate that under short photoperiod and weak light intensity all RhTPP members were significantly down-regulated. Additionally, RhTPS1a, RhTPS10, and RhTPS11 were up-regulated under a short photoperiod and showed a negative correlation with flowering time and trehalose content decrease. Under weak light intensity, RhTPS11 was up-regulated and negatively regulated flowering, while RhTPS5, RhTPS6, RhTPS7b, RhTPS9, and RhTPS10 were down-regulated and positively regulated flowering. This work lays the foundation for revealing the functions of RhTPS and RhTPP gene families in the regulation of rose trehalose.
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DOI：
10.1007/978-1-4614-0634-1_14
发表时间：
2012-01-01
期刊：
ABIOTIC STRESS RESPONSES IN PLANTS: METABOLISM, PRODUCTIVITY AND SUSTAINABILITY
影响因子：
--
作者：
Lopez-Gomez, Miguel;Lluch, Carmen
通讯作者：
Lluch, Carmen
影响因子：
6.6
作者：
Krasensky, Julia;Broyart, Caroline;Jonak, Claudia
通讯作者：
Jonak, Claudia
6-磷酸海藻糖合酶基因 OsTPS1 的过度表达增强了水稻的非生物胁迫耐受性
影响因子：
4.3
作者：
Li, Hao-Wen;Zang, Bai-Sheng;Wang, Xi-Ping
通讯作者：
Wang, Xi-Ping
杨属海藻糖-6-磷酸合酶和海藻糖-6-磷酸磷酸酶基因家族的特征和表达模式。
DOI：
10.1016/j.ijbiomac.2021.07.096
发表时间：
2021-07-22
影响因子：
8.2
作者：
Gao, Yuhan;Yang, Xiaoyu;Chen, Zhong
通讯作者：
Chen, Zhong
影响因子：
5.3
作者：
Bledsoe SW;Henry C;Griffiths CA;Paul MJ;Feil R;Lunn JE;Stitt M;Lagrimini LM
通讯作者：
Lagrimini LM